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  • 53702
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53702

Millipore

Pronase® Protease, Streptomyces griseus

Synonym(s):
Pronase® powder
CAS Number:
MDL number:

form

lyophilized

Quality Level

specific activity

≥45,000 proteolytic units/g dry wt

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)

solubility

water: 10 mg/mL

shipped in

ambient

storage temp.

2-8°C

General description

A non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but it is stable over a wide pH and temperature range. Has an optimal pH of 7.5. Note: 1 KU = 1000 units.
A non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but stable over wide range of pH and temperature. Note: 1KU = 1000 units.
Note: 1 KU = 1000 units.

Packaging

10 , 25 , 250 , 50 , 500 ku in Plastic ampoule
3300, 3500, 5000 KU
1 mu

Warning

Toxicity: Harmful (C)

Unit Definition

One unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.

Physical form

Lyophilized from a solution containing calcium acetate as a stabilizer.

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for 24 h at 4°C.

Other Notes

Narahashi, Y., et al. 1968. J. Biochem. 64, 427.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PRONASE is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Certificate of Analysis

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Certificate of Quality

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