The concentration is 50 µg/ml in PBS, pH 7.4.
537060
Propidium Iodide Solution
A convenient form of propidium iodide useful for flow cytometry studies.
Synonym(s):
Propidium Iodide Solution
Sign In to View Organizational & Contract Pricing.
About This Item
Empirical Formula (Hill Notation):
C27H34I2N4
CAS Number:
Molecular Weight:
668.39
UNSPSC Code:
12352200
NACRES:
NA.47
MDL number:
Skip To
Product Name
Propidium Iodide Solution, A convenient form of propidium iodide useful for flow cytometry studies.
SMILES string
[I-].I.[N+](CCCN1C2=CC(=N)C=CC2=C3C(=C1c4ccccc4)C=C(C=C3)N)(CC)(CC)C
InChI
1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
InChI key
XJMOSONTPMZWPB-UHFFFAOYSA-M
description
RTECS - SF7949600
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
protect from light
input
sample type intact cells
fluorescence
λex 536 nm
λem 617 nm
shipped in
ambient
storage temp.
2-8°C
Quality Level
Related Categories
1 of 4
This Item | 537059 | P4864 | P4170 |
|---|---|---|---|
| form liquid | form solid | form liquid | form powder |
| Quality Level 100 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
| manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® | manufacturer/tradename - | manufacturer/tradename - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| storage condition OK to freeze, protect from light | storage condition OK to freeze, protect from light | storage condition - | storage condition - |
| shipped in ambient | shipped in ambient | shipped in - | shipped in - |
Biochem/physiol Actions
Product does not compete with ATP.
Reversible: no
Cell permeable: no
Primary Target
Membrane impermeable DNA intercalator
Membrane impermeable DNA intercalator
Disclaimer
Toxicity: Standard Handling (A)
General description
Membrane impermeant DNA intercalator. Useful for staining apoptotic cells and nuclei by flow cytometry. Useful in differentiating between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes, e.g. fluorescein isothiocyanate. Excitation max.: ~536 nm; Emission max.: ~617 nm.
Other Notes
Belloc, F., et al. 1994. Cytometry17, 59.
Cropmton, T., et al. 1992. Biochem. Biophys. Res. Commun.183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992. Cytometry13, 432.
Cropmton, T., et al. 1992. Biochem. Biophys. Res. Commun.183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992. Cytometry13, 432.
Physical form
50 µg/ml in PBS, pH 7.4.
Preparation Note
Following initial use, aliquot and freeze (-20°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Protocols
Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.
-
what is the concentration of the PI solution
1 answer-
Helpful?
-
-
What is the recommended protocol for use/ working concentration?
1 answer-
The working concentration must be determined by the end user.
In the following reference: Cytometry. 1994 Sep 1;17(1):59-65. PMID: 7528124 DOI: 10.1002/cyto.990170108; 5ug/ML of PI was directly added in the culture medium for 30min. at 37C.Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service