MilliporeSigma
  • 537088
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537088

Millipore

Pronase® Protease, Streptomyces griseus, Nuclease-Free

Widely used broad-spectrum protease mixture

All Photos(1)
CAS Number:
9036-06-0
MDL number:
MFCD00132092

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form

lyophilized

specific activity

≥70,000 proteolytic units/g dry wt

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

solubility

water: 10 mg/mL

foreign activity

Nuclease, none detected

shipped in

ambient

storage temp.

2-8°C

General description

PRONASE® Protease, Streptomyces griseus, nuclease-free is a non-specific protease that liquefies mucins and digests proteins to free amino acids. This mixture can be used to isolate living chondrocytes. It is most active at pH 7.0 but stable over wide ranges of pH and temperature. It has an optimal pH of 7.5. Pronase is less cytotoxic and produces more efficient dissociation than collagenase.

Application

Pronase® Protease, Streptomyces griseus, nuclease-free has been used:
  • in single-cell preparations for cardiac side population (CSP) analysis
  • in aflatoxin plasma biomarker assay
  • to permeabilize Caenorhabditis elegans embryo for in vivo micrococcal nuclease digestion

Packaging

25 ku in Glass bottle
10 , 100 , 5 , 50 ku in Plastic ampoule

Biochem/physiol Actions

Proteases catalyze the hydrolysis of proteins to form peptides and free amino acids. They also regulate the breakdown of fats and carbohydrates through many enzymatic cascades and activating proenzyme zymogens.

Warning

Toxicity: Harmful (C)

Unit Definition

One unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.

Reconstitution

Unstable in solution; reconstitute just prior to use.

Other Notes

Note: 1 KU = 1000 units.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PRONASE is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation markHealth hazard
GHS07,GHS08

Signal Word

Danger

Hazard Statements

H315 - H319 - H334 - H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Certificate of Analysis

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Certificate of Quality

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