One ml is sufficient for approximately 20 g tissue. Alternatively, depending on the application, it can be used effectively with a dilution factor in the range of 1:100-1:2000. The optimal working concentration should be determined for each individual application. A dilution of 1:200 typically works well as a starting concentration. A list of components with the corresponding concentrations are provided below.
539134
Protease Inhibitor Cocktail III
EDTA-Free, liquid, for the inhibition of aspartic, cysteine, and serine proteases and aminopeptidases in mammalian cells and tissues.
Synonym(s):
EDTA free Protease inhibitor, EDTA-Free Protease Inhibitor Mix, Protease inhibitor cocktail III
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1 ML
$114.00
1 SET
$482.00
10 ML
$656.00
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1 ML
$114.00
1 SET
$482.00
10 ML
$656.00
About This Item
UNSPSC Code:
12352200
NACRES:
NA.77
form:
liquid
solubility:
water: soluble
storage temp.:
−20°C
Product Name
Protease Inhibitor Cocktail Set III, EDTA-Free, Protease inhibitor cocktail III, EDTA-free for inhibiting aspartic, cysteine, and serine proteases as well as aminopeptidases in mammalian cells and tissues.
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
solubility
water: soluble
shipped in
wet ice
storage temp.
−20°C
Related Categories
1 of 4
This Item | 539137 | 535140 | 535142 |
|---|---|---|---|
| form liquid | form lyophilized | form liquid | form lyophilized |
| manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| storage condition OK to freeze | storage condition OK to freeze, desiccated (hygroscopic) | storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition OK to freeze, desiccated (hygroscopic) |
General description
Note: available as 1 x 1 ml or as a set of 5 x 1 ml.
This protease inhibitor cocktail set is a specially formulated cocktail of six protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases. This cocktail is recommended for use with mammalian cells and tissue extracts, but is also suitable for bacterial extracts. It is provided as a single vial or as a set of 5 vials. Each vial contains 1 ml protease inhibitor cocktail solubilized in DMSO with a small amount of water.
Specificity
Inhibits a broad spectrum of serine proteases, cysteine proteases, and aspartic proteases, as well as aminopeptidases.
Application
Protease Inhibitor Cocktail Set III, EDTA-Free has been used:
- as a component in CelLytic MT cell lysis reagent or lysis buffer to homogenize kidney organoids for immunoblot analysis[1]
- as a component in homogenization buffer to homogenize minced mouse quadriceps femoris muscle tissue[2]
- in lysis buffer to extract proteins for immunoblotting[3]
Biochem/physiol Actions
Protease inhibitor cocktails and specialized protease inhibitors play a crucial role in various proteomic studies conducted by scientists. These studies encompass protein expression and characterization, biomarker discovery, identification of post-translational modifications, protein expression profiling, and quantitative protein measurement. The inclusion of a protease inhibitor cocktail or specific protease inhibitor guarantees the preservation of protein integrity, allowing for accurate downstream analysis and comprehensive sample characterization.
Features and Benefits
- EDTA-Free: recommended for IMAC applications.
- Flexible: compatible with bacterial extracts, mammalian cell extracts, and tissue extracts.
- Fast: no waiting time for tablets to dissolve.
- Economical: ready-to-use format reduces wastage.
- Effective: one ml is sufficient for approximately 20g of tissue.
- Complete: contains six protease inhibitors to inhibit aspartic, cysteine, serine proteases, and aminopeptidases.
Warning
Toxicity: Irritant (B)
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
188.6 °F - (refers to pure substance)
flash_point_c
87 °C - (refers to pure substance)
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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is this cocktail used as 1000X or 100X?
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