539149

Proteasome Substrate VI, Fluorogenic

A fluorogenic substrate probe useful for assaying trypsin-like activity of proteasome.

• Synonym(s): Proteasome Substrate VI, Fluorogenic, Z-ARR-AMC, 2HCl
• Empirical Formula (Hill Notation): C₃₃H₄₄N₁₀O₇ · 2HCl
• CAS Number: 90468-18-1
• Molecular Weight: 765.69
• MDL number: MFCD00011127
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• assay: ≥98% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: white to off-white
• solubility: DMSO: 200 mg/mL; 80% acetic acid: 50 mg/mL; methanol: soluble
• fluorescence: λ_ex ~380 nm; λ_em ~460 nm
• shipped in: ambient
• Storage temp.: −20°C

Description

General description

A fluorogenic substrate for assaying trypsin-like activity of proteasome. Also serves as a substrate for cathepsin B. Excitation max: 360-380 nm; emission max.: 430-460 nm.

A fluorogenic substrate probe useful for assaying trypsin-like activity of proteasome. Also reported to be a specific substrate for cathepsin B.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Proteasome

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

Z-Ala-Arg-Arg-AMC

Physical form

Supplied as hydrochloride salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 3 months at -20°C

Other Notes

Geier, E., et al. 1999. Science283, 978.
Dick, T.P., et al. 1998. J. Biol. Chem. 273, 25637.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable