539161

Proteasome Inhibitor I

InSolution, ≥90%

• Synonym(s): InSolution Proteasome Inhibitor I, PSI
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• assay: ≥90% (HPLC)
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic); protect from light
• storage temp.: −20°C

Description

General description

Cell-permeable inhibitor of the chymotrypsin-like activity of the multicatalytic proteinase complex (MCP; 20S proteasome) in HT4 cells. Causes the accumulation of ubiquitinated proteins in neuronal cells. Prevents NF-κB activation in response to TNFα and okadaic acid through inhibition of IκBα degradation, thereby interfering with induction of iNOS in macrophages.

Biochem/physiol Actions

Primary Target
chymotrypsin-like activity of the multicatalytic proteinase complex (MCP; 20S proteasome) in HT4 cells

Product does not compete with ATP.

Packaging

Packaged under inert gas

Warning

Toxicity: Irritant (B)

Sequence

Z-Ile-Glu(OtBu)-Ala-Leu-CHO

Physical form

A 50 mM (5 mg/162 µl) solution of Proteasome Inhibitor I (Cat. No. 539160) in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Griscavage, J.M., et al. 1996. Proc. Natl. Acad. Sci. USA93, 3308.
Mori, S., et al. 1995. J. Biol. Chem.270, 29447.
Figueiredo-Pereira, M.E., et al. 1994. J. Neurochem.63, 1578.
Traenckner, E.B., et al. 1994. EMBO J.13, 5433."

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: 188.6 °F - (refers to pure substance)
• flash_point_c: 87 °C - (refers to pure substance)