539180

Millipore

ProteoExtract® Protein Precipitation Kit

storage conditions

+15C to +30C

usage

sufficient for 200 precipitation(s)

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

shipped in

ambient

storage temp.

10-30°C

General description

A convenient and highly reproducible kit for the precipitation and clean-up of proteins. Yield of precipitated proteins is higher than that of traditional methods with improved solubility of the precipitated protein. Contains sufficient components to precipitate 200 samples of ≤200 µl. Requires 150 ml of ethanol (not provided). Kit delivers protein solutions of very low conductivity.

Components

Precipitant 1, Precipitant 2, Precipitant 3, Precipitant 4, Wash Solution, and a user protocol.

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Principle

The ProteoExtract Protein Precipitation Kit is designed for the concentration and clean-up of proteins from aqueous samples.

Preparation Note

Note: Prior to using the kit for the first time, the Precipitation Agent and the Wash Solution must be prepared and chilled to -20°C.• Precipitation Agent: 1. To one bottle Precipitant 1 add 1.7 ml Precipitant 2, 1.7 ml Precipitant 3, and 1.7 ml Precipitant 4; mix well.2. The mixture of Precipitant 1, 2, 3, and 4 is called the Precipitation Agent. One bottle Precipitation Agent (34.1ml) is sufficient for precipitation of 40 samples of 200 µl each. 3. Label the Precipitant 1 bottle after reconstitution for easy recognition. For ease of use, store the Precipitation Agent at -20°C; reconstituted Precipitation Agent is stable for up to 2 months.• Wash Solution:1. Add 150 ml high quality ethanol (not provided) to the bottle of Wash Solution (denatured ethanol may also be used).2. Mix well and store at -20°C. Reconstituted Wash Solutions is stable for up to 1 year.Note: Each 200 µl sample requires 800 µl Precipitation Agent and 1 ml Wash Solution.
• Samples: The assay will precipitate proteins from a broad range of sample types containing from 50 µg/ml to 10 mg/ml protein. The assay will tolerate chaotropes (e.g., UREA) to to a concentration of 8 M, detergents up to 4%, and salts up to 2 M.

Storage and Stability

Upon arrival, all components of the kit can be stored at room temperature (20°C). For ease of use, the prepared Precipitation Agent and the Wash Solution can be stored at -20°C (see Reagent Preparation).

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PROTEOEXTRACT is a registered trademark of Merck KGaA, Darmstadt, Germany
Proliferating infantile hemangioma promotes α-fetoprotein production by HepG2 cells.
Bede van Schaijik et al.
Pediatric research, 87(1), 3-6 (2019-08-15)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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