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539568

Sigma-Aldrich

PP2B, Human, Recombinant, E. coli

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Synonym(s):
CaN, Protein Phosphatase 2B, Calcineurin

form

liquid

Quality Level

specific activity

≥300,000 U/mg
≥50,000 units/mL

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

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This Item
218701218785539735
specific activity

≥300,000 U/mg, ≥50,000 units/mL

specific activity

≥500,000 units/mL

specific activity

≥150,000 units/mg protein, ≥50,000 units/mL

specific activity

≥20 U/mg

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

General description

Recombinant, human calcineurin Aα (catalytic subunit) and calcineurin B (regulatory subunit) co-expressed with yeast myristoyl-CoA:protein N-myristoyltransferase. A Ca2+/CaM-dependent protein phosphatase. The resulting highly active calcineurin is N-myristoylated on the CaN B-subunit, as is the native protein. M.W. 60,000 (A subunit) and 15,000 (B subunit).

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will release 1.0 pmol of phosphate from the RII phosphopeptide substrate (DLDVPIPGRFDRRVpSVAAE; Cat. No. 207008) in the presence of calmodulin per min at 30°C, pH 7.4.

Physical form

In 100 mM NaCl, 50 mM Tris, 6 mM MgCl₂, 5 mM DTT, 0.5 mM CaCl₂, 0.025% NP-40.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C). Please refer to vial label for lot-specific reconstitution volume.

Analysis Note

Two distinct bands by SDS-PAGE

Other Notes

Guerini, D. 1997. Biochem. Biophys. Res. Commun. 235, 271.
Mondragon, A., et al. 1997. Biochemistry 36, 4934.
Wei, Q. and Lee, E.Y. 1997. Biochemistry36, 7418.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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