539638

PPI2, Human, Recombinant, E. coli

• Synonym(s): Protein Phosphatase Inhibitor 2
• UNSPSC Code: 12352200

Properties

• assay: ≥95% (SDS-PAGE)
• Quality Level: 100
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic)
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Recombinant, human PPI2 expressed in E. coli. Inhibitor-2 is the heat-stable, regulatory subunit of the cytosolic type 1 Ser/Thr protein phosphatase-1 (PP1). Inhibits the activity of the free catalytic subunit of PP1 (IC₅₀ = 1 nM). Inhibition is reported to involve a "masking region" on the inhibitor that lies in close proximity to the active site of PP1 and blocks the access to substrates. Interaction of inhibitor-2 with the catalytic subunit of PP1 leads first to a rapid inhibition of the enzyme activity followed by the formation of a stable inactive complex. The inactive complex can be reactivated through phosphorylation of inhibitor-2 at Thr⁷² by glycogen synthase kinase-3 (GSK-3).

Biochem/physiol Actions

Cell permeable: no

Primary Target
activity of free catalytic subunit of PP1

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 1 nM inhibiting the activity of the free catalytic subunit of PP1

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C).

Reconstitute with 50 mM Tris-HCl, pH 7.0.

Other Notes

Yang, J., et al. 2000. J. Biol. Chem.275, 22635.
Huang, H.B., et al. 1999. J. Biol. Chem.274, 7870.
Helps, N.R., et al. 1994. FEBS Lett.340, 93.
Park, I., et al. 1994. J. Biol. Chem.269, 944.
Cohen, P. 1991. Methods Enzymol.201, 389.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable