Recombinant, human PPI2 expressed in E. coli. Inhibitor-2 is the heat-stable, regulatory subunit of the cytosolic type 1 Ser/Thr protein phosphatase-1 (PP1). Inhibits the activity of the free catalytic subunit of PP1 (IC50 = 1 nM). Inhibition is reported to involve a "masking region" on the inhibitor that lies in close proximity to the active site of PP1 and blocks the access to substrates. Interaction of inhibitor-2 with the catalytic subunit of PP1 leads first to a rapid inhibition of the enzyme activity followed by the formation of a stable inactive complex. The inactive complex can be reactivated through phosphorylation of inhibitor-2 at Thr72 by glycogen synthase kinase-3 (GSK-3).
Biochem/physiol Actions
Cell permeable: no
Primary Target activity of free catalytic subunit of PP1
Product does not compete with ATP.
Reversible: no
Target IC50: 1 nM inhibiting the activity of the free catalytic subunit of PP1
Warning
Toxicity: Standard Handling (A)
Reconstitution
Following reconstitution, aliquot and freeze (-20°C).
Reconstitute with 50 mM Tris-HCl, pH 7.0.
Other Notes
Yang, J., et al. 2000. J. Biol. Chem.275, 22635. Huang, H.B., et al. 1999. J. Biol. Chem.274, 7870. Helps, N.R., et al. 1994. FEBS Lett.340, 93. Park, I., et al. 1994. J. Biol. Chem.269, 944. Cohen, P. 1991. Methods Enzymol.201, 389.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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