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MilliporeSigma

540221

Sigma-Aldrich

Purine Nucleoside Phosphorylase, Human, Recombinant, E. coli

Synonym(s):

PNP

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.42

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recombinant

expressed in E. coli

Quality Level

100

form

liquid

specific activity

≥25 units/mg protein

manufacturer/tradename

Calbiochem®

shipped in

dry ice

storage temp.

−20°C

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Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide.

Sigma-Aldrich

324717

Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli

specific activity

≥25 units/mg protein

specific activity

≥20 U/mg

specific activity

≥1400 units/mg protein (after activation)

specific activity

≥3 units/mL, ≥30 units/mg protein

form

liquid

form

liquid

form

liquid

form

liquid

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

-

recombinant

expressed in E. coli

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

2-8°C

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

General description

Recombinant, human purine nucleotide phosphorylase expressed in E. coli without tags. Purine nucleoside phosphorylase catalyzes the phosphorlysis of N-ribosidic bonds of purine nucleosides and deoxynucleosides in a reversible reaction. Human PNP is a target for therapeutic T-cell immune response intervention. Useful in the detection of inorganic phosphate from biochemical reactions.

Physical form

In 50 mM Tris-HCl, pH 7.6.

Analysis Note

Single band by SDS-PAGE

Other Notes

Filgueira de Azevedo, W.Jr., et al. 2003. Biochem. Biophys. Res. Commun.309, 917.
Silva, R.G., et al. 2003. Protein Expr. Purif.27, 158.
One unit is defined as the amount of enzyme that will convert 1 µmol of MESG and 1 µmol of Pi into 1 µmol of 2-amino-6-mercapto-7-methylpurine and 1 µmol of ribose phosphate in 1 min at 25°C, pH 7.6.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Roman S Iwasaki et al.
Nucleic acids research, 48(17), e101-e101 (2020-08-17)
Recent efforts in biological engineering have made detection of nucleic acids in samples more rapid, inexpensive and sensitive using CRISPR-based approaches. We expand one of these Cas13a-based methods to detect small molecules in a one-batch assay. Using SHERLOCK-based profiling of

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