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552101

Sigma-Aldrich

Anti-Rab9 Mouse mAb (mab9)

liquid, clone mab9, Calbiochem®

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

antibody form

diluted ascites fluid

antibody product type

primary antibodies

clone

mab9, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, bovine, canine, rat, hamster, monkey

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG1

shipped in

ambient

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RAB9A(9367)

General description

Mouse monoclonal antibody supplied as diluted ascites. Recognizes the ~26 kDa Rab9 protein.
This Anti-Rab9 Mouse mAb (mab9) is validated for use in Immunoblotting, Immunofluorescence, Immunohistochemistry, Immunoprecipitation for the detection of Rab9.

Immunogen

recombinant, canine Rab9

Application

Immunoblotting (1:1000)

Immunofluorescence (see comments)

Immunohistochemistry (1:500)

Immunoprecipitation (see comments)

Warning

Toxicity: Standard Handling (A)

Physical form

Ascites diluted in PBS.

Other Notes

Reacts with both prenylated and non-prenylated Rab9. Does not cross-react with other Rab family members. This antibody has also been reported to work for immunofluorescence and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Schimmoller, F., et al. 1998. J. Biol. Chem.273, 22161.
Shapiro, A. and Pfeffer, S. 1995. J. Biol. Chem.270, 11085.
Pfeffer, S. 1993. Mol. Biol. Cell4, 425.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Sang H Lee et al.
Nature communications, 9(1), 3434-3434 (2018-08-26)
Secreted Wnts play crucial roles in synaptogenesis and synapse maintenance, but endogenous factors promoting synapse elimination in central neurons remain unknown. Here we show that proline-rich 7 (PRR7) induces specific removal of excitatory synapses and acts as a Wnt inhibitor.

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