553571

Sigma-Aldrich

Anti-Ras (31-43) Rabbit pAb

liquid, Calbiochem®

Synonym(s):
Anti-Ras GDP/GTP Binding Protein
Pricing and availability is not currently available.

storage conditions

-20C

biological source

rabbit

Quality Level

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, mouse, rat, bovine

mfr. no.

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

General description

Recognizes all forms of wild type Ras, including the mutant G12V Ras protein.
Immunoaffinity rabbit polyclonal antibody. Recognizes all forms of wild type Ras and the mutant G12V Ras protein.
This Anti-Ras (31-43) Rabbit pAb is validated for use in Immunoblotting for the detection of Ras (31-43).

Immunogen

a synthetic peptide [EYDPTIEDSYRKQ(C)] corresponding to amino acids 31-43 in the effector binding Loop (L2) of human H-, K-, and N-Ras, conjugated to KLH

Packaging

50 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Analysis Note

Positive Control
Rat brain tissue

Other Notes

Hardie, G. and Hanks, S. 1995. in The Protein Kinase Facts Book, Protein-Serine Kinases p. 418,Academic Press, San Diego, CA.
Sawada, M., et al. 1989. Nucleic Acid Res.17, 8867.
By immmunoblotting, this anti-pan-Ras polyclonal antibody reacts strongly with recombinant Ras fusion protein as well as with recombinant glutathione S-transferase (GST) human Ras and [G12V]GST human Ras. Does not cross-react with members of the Rho or Rab families of Ras-related small G-proteins. No specific reactivity is detected by immunoblotting with rat, mouse or human cell lines or tissue extracts. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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