555466

Rho, GST-Fusion, Human, Recombinant, E. coli

• UNSPSC Code: 12352202

Properties

• assay: ≥90% (SDS-PAGE)
• Quality Level: 100
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• capacity: ≥400 mmol/mol binding capacity (GTPγS)
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Recombinant, human Rho fused to GST and expressed in E. coli. A small GTPase of the Ras superfamily. Regulates stress fiber formation in response to growth factor stimulation. Useful as a positive control in immunoblotting analysis and as an affinity precipitation reagent using immobilized glutathione resin.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 20 mM HEPES, 5 mM MgCl₂, 40% glycerol, 0.05% NaN₃, pH 8.0.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Ridley, A. 1996. Curr. Biol. 6, 1256.
Ridley, A. and Hall, A. 1992. Cell 70, 389.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable