556881

Ribonuclease Inhibitor, Human, Recombinant, E. coli - Calbiochem

The Ribonuclease Inhibitor, Human, Recombinant, E. coli controls the biological activity of Ribonuclease. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.

Properties

• Quality Level: 100
• form: liquid
• specific activity: ≥20,000 units/mL
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• foreign activity: Endonuclease and RNase, none detected
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Recombinant, human ribonuclease inhibitor expressed in E. coli. Non-competitive inhibitor that inactivates RNase by non-covalent binding. Has been used to improve cDNA synthesis and in vitro RNA synthesis, increase yields of polysomes and aid in the preparation of RNase-free antibodies. Inhibits RNases A, B, and C. Does not inhibit RNase T₁ and S1 nuclease from Aspergillus.

Recombinant, human ribonuclease inhibitor expressed in E. coli. Non-competitive inhibitor that inactivates RNase by non-covalent binding. Has been used to improve cDNA synthesis and in vitro RNA synthesis, increase yields of polysomes, and aid in the preparation of RNase-free antibodies. Inhibits RNases A, B, and C. Does not inhibit RNase T₁ and S1 nuclease from Aspergillus.

Packaging

2500 u in Plastic ampoule

Biochem/physiol Actions

Product does not compete with ATP.

Reversible: no

Cell permeable: no

Primary Target
inactivates RNase

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of material that will inhibit 50% of the activity of 5 ng of RNase A.

Physical form

In 50 mM KCl, 20 mM HEPES, 8 mM DTT, 50% glycerol.

Reconstitution

Maintain at -20°C. Avoid freezing solutions. Addition of DTT is recommended to maintain 8 mM level.

Other Notes

Saxena, S.K., et al. 1992. J. Biol. Chem. 267, 21982.
Lee, F.S., et al. 1989. Biochemistry 28, 225.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable