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556881

Sigma-Aldrich

Ribonuclease Inhibitor, Human, Recombinant, E. coli - Calbiochem

The Ribonuclease Inhibitor, Human, Recombinant, E. coli controls the biological activity of Ribonuclease. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.

Quality Level

form

liquid

specific activity

≥20,000 units/mL

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

foreign activity

Endonuclease and RNase, none detected

shipped in

wet ice

storage temp.

−20°C

General description

Recombinant, human ribonuclease inhibitor expressed in E. coli. Non-competitive inhibitor that inactivates RNase by non-covalent binding. Has been used to improve cDNA synthesis and in vitro RNA synthesis, increase yields of polysomes and aid in the preparation of RNase-free antibodies. Inhibits RNases A, B, and C. Does not inhibit RNase T1 and S1 nuclease from Aspergillus.
Recombinant, human ribonuclease inhibitor expressed in E. coli. Non-competitive inhibitor that inactivates RNase by non-covalent binding. Has been used to improve cDNA synthesis and in vitro RNA synthesis, increase yields of polysomes, and aid in the preparation of RNase-free antibodies. Inhibits RNases A, B, and C. Does not inhibit RNase T1 and S1 nuclease from Aspergillus.

Packaging

2500 u in Plastic ampoule

Biochem/physiol Actions

Product does not compete with ATP.
Reversible: no
Cell permeable: no
Primary Target
inactivates RNase

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of material that will inhibit 50% of the activity of 5 ng of RNase A.

Physical form

In 50 mM KCl, 20 mM HEPES, 8 mM DTT, 50% glycerol.

Reconstitution

Maintain at -20°C. Avoid freezing solutions. Addition of DTT is recommended to maintain 8 mM level.

Other Notes

Saxena, S.K., et al. 1992. J. Biol. Chem. 267, 21982.
Lee, F.S., et al. 1989. Biochemistry 28, 225.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

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