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Sigma-Aldrich

Sphingolipid Ceramide N-Deacylase, Pseudomonas sp.

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Synonym(s):
SCDase

biological source

bacterial (Pseudomonas spp.)

Quality Level

form

liquid

specific activity

≥1 units/mL

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

foreign activity

α- and β-N-acetylgalactosaminidase, α-fucosidase, α- and β-galactosidase, neuraminidases, none detected

shipped in

wet ice

storage temp.

−20°C

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Sigma-Aldrich

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Elastase, Pseudomonas aeruginosa

biological source

bacterial (Pseudomonas spp.)

biological source

-

biological source

-

biological source

bacterial (Elizabethkingia meningosepticum)

form

liquid

form

-

form

lyophilized

form

liquid

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

-

storage condition

OK to freeze

storage condition

do not freeze

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

shipped in

wet ice

shipped in

dry ice

shipped in

ambient

shipped in

wet ice

General description

Sphingolipid ceramide N-deacylase (SCDase) is a native sphingolipid ceramide N-deacylase from Pseudomonas species. It hydrolyzes the N-acyl linkage between fatty acids and the sphingosine base of ceramide in several sphingolipids and gangliosides. This enzyme catalyzes the condensation reaction between fatty acids and sphingosine bases and forms sphingolipids. It is also involved in transacylation reactions. SCDase is not recommended for hydrolysis of ceramide. It is also used to prepare lysoglycosphingolipids (lysoGSLs) effortlessly.

Application

Sphingolipid ceramide N-Deacylase, Pseudomonas sp. has been used as a PP2A enhancer to study its effects on phosphatase and tensin homolog (PTEN) in forkhead box O3 (FOXO3a) phosphorylation in primary CD4+ T cells.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of asialo-GM₁ per min at 37°C, pH 6.0.

Physical form

In 50 mM sodium acetate buffer, 0.1% LUBROL PX Detergent, pH 6.0.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Mitsutake, S., et al. 1997. Anal. Biochem. 247, 52.
Sueyoshi, N., et al. 1997. J. Lipid Res. 38, 1923.
Ito, M., et al. 1995. J. Biol. Chem. 270, 24370.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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S Mitsutake et al.
Analytical biochemistry, 247(1), 52-57 (1997-04-05)
Sphingolipid ceramide N-deacylase (SCDase) is an enzyme which hydrolyzes the N-acyl linkage between fatty acid and sphingosine in ceramide of various glycosphingolipids and sphingomyelin. Recently the enzyme was found to catalyze the hydrolysis and its reverse reaction under different conditions.
Nayoung Kim et al.
PLoS pathogens, 6(9), e1001103-e1001103 (2010-09-24)
Apoptosis in HIV-1-infected CD4+ primary T cells is triggered by the alteration of the PI3K and p53 pathways, which converge on the FOXO3a transcriptional activator. Tat alone can cause activation of FOXO3a and of its proapoptotic target genes. To understand
T Kurita et al.
Journal of lipid research, 41(5), 846-851 (2000-04-29)
Lysoglycosphingolipids were produced from glycosphingolipids by using sphingolipid ceramide N-deacylase, which cleaves the N-acyl linkage between fatty acids and sphingosine bases in various glycosphingolipids. The enzyme reaction was done in a biphasic media prepared with water;-immiscible organic solvent and aqueous

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