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574797

TAE Buffer, 10X, Molecular Biology Grade

A 10X concentrate that can be diluted to a 1X solution containing 40 mM Tris, 40 mM acetate, and 1 mM EDTA, pH ~8.3.

Synonym(s):

Tris-Acetate-EDTA Buffer

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1 L
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$134.00

About This Item

NACRES:
NA.25
UNSPSC Code:
12161700

$134.00

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form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, desiccated

technique(s)

electrophoresis: suitable

foreign activity

DNase, RNase, protease, none detected

shipped in

ambient

storage temp.

15-25°C

General description

Tris, acetate, and ethylenediaminetetraacetic acid (TAE) buffer consists of tris(2-amino-2-[hydroxymethyl]-1,3-propanediol) base, acetate, and ethylenediaminetetraacetic acid (EDTA). This is one of the most used running buffers in nucleic acid electrophoresis. TAE buffer improves the separation or resolution of large DNA fragments.[1]TAE, a standard buffer, is mainly used to perform agarose gel electrophoresis of DNA and RNA in slab gels. TAE is one of the commonly used buffers in every biochemistry and molecular biology laboratory.

Application

TAE Buffer, 10X, Molecular Biology Grade - Calbiochem has been used in 1X concentration for the preparation of agarose gel and as a running buffer in gel electrophoresis.[2]

Preparation Note

Dilute 1:10 with H₂O to prepare a 1X solution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

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TAE Buffer, 10X, Molecular Biology Grade A 10X concentrate that can be diluted to a 1X solution containing 40 mM Tris, 40 mM acetate, and 1 mM EDTA, pH ~8.3.

Sigma-Aldrich

574797

TAE Buffer, 10X, Molecular Biology Grade

TE Buffer, 100X, Molecular Biology Grade A 100X concentrate that, when diluted to 1X, contains 10 mM Tris, 1 mM EDTA, pH ~8.0.

Sigma-Aldrich

574793

TE Buffer, 100X, Molecular Biology Grade

TBE Buffer, 10X, Molecular Biology Grade A 10X concentrate that can be diluted to a 1X solution containing 89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH ~8.3.

Sigma-Aldrich

574795

TBE Buffer, 10X, Molecular Biology Grade

Tris Acetate-EDTA buffer pH 8.3, pHast Pack™, powder

Sigma-Aldrich

PPB008

Tris Acetate-EDTA buffer

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

-

technique(s)

-

form

liquid

form

liquid

form

liquid

form

powder

storage condition

OK to freeze, desiccated

storage condition

OK to freeze, desiccated

storage condition

OK to freeze

storage condition

-

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

-

foreign activity

DNase, RNase, protease, none detected

foreign activity

DNase, RNase, protease, none detected

foreign activity

DNase, RNase, protease, none detected

foreign activity

DNAse, none detected, Nickase, none detected, Protease, none detected, RNAse, none detected

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

-

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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