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605160

Sigma-Aldrich

Thrombin, Plasminogen-Free, Bovine

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Synonym(s):
Thrombin, Plasminogen-Free, Bovine
CAS Number:
9002-04-4

form

lyophilized

Quality Level

100

specific activity

≥1000 NIH units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated

solubility

water: 1 mg/mL

foreign activity

Plasminogen, none detected (by rocket IEP)

shipped in

ambient

storage temp.

−20°C

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This Item
T73266051571.12374
Sigma-Aldrich

Sigma-Aldrich

605160

Thrombin, Plasminogen-Free, Bovine

Thrombin from bovine plasma ≥60 NIH units/mg protein (biuret)

Sigma-Aldrich

T7326

Thrombin from bovine plasma

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Sigma-Aldrich

605157

Thrombin, Bovine

Sigma-Aldrich

Sigma-Aldrich

1.12374

Thrombin

specific activity

≥1000 NIH units/mg protein

specific activity

≥60 NIH units/mg protein (biuret)

specific activity

≥1800 NIH units/mg protein, ≥50 NIH units/mg dry wt

specific activity

50 NIH units/mg dry wt

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

storage condition

OK to freeze, desiccated

storage condition

-

storage condition

OK to freeze

storage condition

-

solubility

water: 1 mg/mL

solubility

-

solubility

0.05 M NaCl: ≤2000 NIH units/mL, water: >2000 NIH units/mL

solubility

-

foreign activity

Plasminogen, none detected (by rocket IEP)

foreign activity

-

foreign activity

-

foreign activity

-

General description

Native, bovine thrombin. Key enzyme that activates factor XIII and converts fibronogen into fibrin. Note: 1 KU = 1000 units.
Native, bovine thrombin. Key enzyme that activates factor XIII and converts fibronogen into fibrin. Serine protease formed in the plasma during the coagulation process by activation of prothrombin by a complex of Factor Xa, Factor Va, phospholipid, and Ca2+. Thrombin cleaves Arg-Gly bonds in specific sequences only. The best characterized activity of thrombin is the conversion of fibrinogen to fibrin in the clotting process. Inhibited by AEBSF (Cat. No. 101500) Antithrombin (Cat. No. 169756), PMSF (Cat. No. 52332), and TLCK (Cat. No. 616382). Note: 1 KU = 1000 units.

Warning

Toxicity: Harmful (C)

Unit Definition

One unit is determined by comparison with a standard curve prepared using the Bureau of Biologics standard thrombin.

Physical form

Lyophilized from 200 mM NaCl, 50 mM sodium citrate, 0.1% PEG-8000, pH 6.5.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 6 months at -70°C.

Analysis Note

Complete activation from homogeneous bovine prothrombin by SDS-PAGE

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation markHealth hazard
GHS07,GHS08

Signal Word

Danger

Hazard Statements

H315 - H319 - H334 - H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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