613548

TMB, Insoluble

• Synonym(s): TMB, Insoluble, 3,3ʹ,5,5ʹ-Tetramethylbenzidine
• CAS Number: 54827-17-7
• MDL number: MFCD00007748

Properties

• form: liquid
• Quality Level: 100
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: clearlight brown
• shipped in: ambient
• storage temp.: 2-8°C
• InChI: 1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
• InChI key: UAIUNKRWKOVEES-UHFFFAOYSA-N

Description

General description

Insoluble TMB substrate supplied as a single component system. TMB is the most sensitive chromogenic substrate for detection of horseradish peroxidase (HRP)-labeled probes. In the presence of HRP and hydrogen peroxide (H₂O₂), TMB is oxidized first to a blue, cation free radical; upon further reaction with HRP/H₂O₂, or addition of acid, the radical is converted to a yellow diimine terminal oxidation product. TMB is used routinely in ELISA procedures, whereby its soluble oxidation products are measured spectrophotometrically. For TMB to be worthwhile as a blotting reagent, enhancers such as dextran sulfate or other sugar polymers are usually added.This TMB reagent is a safe, single component system that has been developed for use in blotting procedures by a proprietary methodology that eliminates the use of sugar polymers. The reagent produces a stable, insoluble aquamarine precipitate at the reaction site with little or no background. It can significantly increase the detection limits of assays on a variety of membranes. Ideal for many immunoblotting procedures and can significantly increase detection limits of assays on a variety of membranes

Peroxidase substrate that produces a stable, insoluble, aquamarine blue precipitate at reaction site with little or no background.

Warning

Toxicity: Carcinogenic / Teratogenic (D)

Physical form

1.19 mM H₂O₂ in a proprietary solvent, 1.13 mM TMB, pH 4.9 ± 0.03.

Reconstitution

Discard if the solution turns blue or turbid. The reagent is stable at room temperature; refrigeration is recommended for long-term storage. Warm to assay temperature before use.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS07,GHS08
• Signal Word: Warning
• Hazard Statements: H302 - H351 - H413
• Precautionary Statements: P202 - P264 - P273 - P280 - P301 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable