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MilliporeSigma

614850

Sigma-Aldrich

Topoisomerase I, His•Tag®, Human, Wild Type, Recombinant, S. frugiperda

Synonym(s):

Topo I, Wild Type

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.54

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recombinant

expressed in insect cells (S. frugiperda)

Quality Level

100

assay

≥95% (SDS-PAGE)

form

liquid

specific activity

1 units/ng protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

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Topoisomerase I, His•Tag®, Human, Wild Type, Recombinant, S. frugiperda

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assay

≥95% (SDS-PAGE)

assay

≥90% (SDS-PAGE)

assay

-

assay

-

specific activity

1 units/ng protein

specific activity

≥10 units/μg protein

specific activity

≥0.5 U/μL

specific activity

≥0.25 U/μL

recombinant

expressed in insect cells (S. frugiperda)

recombinant

expressed in insect cells (S. frugiperda)

recombinant

expressed in insect cells (S. frugiperda)

recombinant

expressed in insect cells (S. frugiperda)

form

liquid

form

liquid

form

liquid

form

liquid

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

General description

Recombinant, human topoisomerase I fused to a His•Tag sequence and expressed in S. frugiperda insect cells. It catalyzes the relaxation of both positive and negative supercoiled DNAs without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA topoisomerase I also plays a role in pre-mRNA splicing, cell cycle and other gene regulatory pathways during cell growth and development. DNA Topo I is an important target for ant-tumor agents as a result of the fact that is causes single-strand DNA breaks of the type that are present in most cancers.

Biochem/physiol Actions

0.1-1 unit (ng) is sufficient for relaxing 0.5 µg of pBR322 supercoiled DNA in a 20 µl reaction.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

5000 U enzyme in 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 200 µM EDTA, 20% glycerol, pH 7.9 and 1 vial dilution buffer (100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0).

Reconstitution

Following initial thaw aliquot and freeze (-70°C). For storage of diluted protein, dilute in storage buffer with 500µg/ml molecular biology grade BSA, aliquot, and freeze (-70°C).

Other Notes

Pommier, Y., et al. 1998. Biochim. Biophys. Acta1400, 83.
Pourquier, P., et al. 1997. J. Biol. Chem.272, 26441.
Rossi, F., et al. 1996. Nature381, 80.
Sekiguchi, J., et al. 1996. Proc. Natl. Acad. Sci. USA93, 785.
Giovanella, B.C., et al. 1989. Science246, 1046.
Wang, J,C. 1985. Annu. Rev. Biochem.54, 665.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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