616376

Sigma-Aldrich

TRAIL, Human, Recombinant, NSO cells

Synonym(s):
Apo2L; TNF-Related Apoptosis-Inducing Ligand

storage conditions

= -70C

Quality Level

assay

≥97% (SDS-PAGE)

form

lyophilized

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

≤1 EU/μg Endotoxin (EU/μg TRAIL)

shipped in

ambient

storage temp.

−70°C

General description

Recombinant, human TRAIL expressed in NSO cells a type II transmembrane protein with a C-terminal extracellular domain, which exhibits homology to other TNF family members. Displays potent antitumor activity against selected targets. Induces apoptosis in a number of transformed cell lines but not in normal tissues.
Recombinant, human TRAIL (amino acids 95-281) with an amino-terminal 6X histidine tag and expressed in NSO cells. A type II transmembrane protein with a C-terminal extracellular domain that exhibits homology to other members of this tumor necrosis factor (TNF) family. Displays potent anti-tumor activity against selected targets. Induces apoptosis in a number of transformed cell lines but not in normal tissues. Recombinant bioactive TRAIL is a non-disulfide-linked homotrimer but may contain a small amount (up to 10%) of disulfide-linked homodimers.

Packaging

10 μg in Plastic ampoule

Biochem/physiol Actions

ED₅₀ = 4-12 ng/ml as measured by its ability to induce cytotoxicity in mouse L-929 cell line in the presence of metabolic inhibitors actinomycin D

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from sterile-filtered PBS, 50 µg BSA/µg TRAIL, pH 7.4.

Reconstitution

Reconstitute to ≥20 µg/ml in sterile PBS, 0.1% BSA.
Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or up to 3 months at -20°C or -70°C. Avoid freeze/thaw cycles of solutions.

Other Notes

Griffith, T.S., et al. 1999. J. Exp. Med.189, 1343.
Mariani, S.M., et al. 1997. J. Cell Biol.137, 221.
Pan, G., et al. 1997. Science277, 815.
Pan, G., et al. 1997. Science276, 111.
Sheridan, J.P., et al. 1997. Science277, 818.
Pitti, R.M., et al. 1996. J. Biol. Chem.271, 12687.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

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