627610

Caspase Inhibitor I

Z-VAD-FMK, CAS 187389-52-2, is a cell-permeable, irreversible, pan-caspase inhibitor.

• Synonym(s): Caspase Inhibitor I, Z-VAD (OMe)-FMK
• Empirical Formula (Hill Notation): C₂₂H₃₀FN₃O₇
• CAS Number: 187389-52-2
• Molecular Weight: 467.49

Properties

• Quality Level: 100
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated
• color: white
• solubility: DMSO: 50 mM
• shipped in: ambient
• storage temp.: −20°C
• InChI: 1S/C22H30FN3O7/c1-13(2)19(26-22(31)33-12-15-8-6-5-7-9-15)21(30)24-14(3)20(29)25-16(17(27)11-23)10-18(28)32-4/h5-9,13-14,16,19H,10-12H2,1-4H3,(H,24,30)(H,25,29)(H,26,31)/t14-,16-,19-/m0/s1
• InChI key: MIFGOLAMNLSLGH-QOKNQOGYSA-N

Description

General description

A cell-permeable, irreversible, pan-caspase inhibitor. Inhibits Fas-mediated apoptosis in Jurkat cells and staurosporine-induced cell death in corneal epithelial cells. Shown to enhance the freeze-thaw survival of human embryonic stem cells. When using with purified native or recombinant enzyme, pre-treatment with an esterase is required.

A cell-permeable, irreversible, pan-caspase inhibitor. Shown to enhance the freeze-thaw survival of human embryonic stem cells. Inhibits Fas-mediated apoptosis in Jurkat T cells. Also reported to inhibit Peptide: N-glycanse (PNGase) in vitro amd in vivo. When using with a purified recombinant enzyme, pretreatment with an esterase is required.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Fas-mediated apoptosis in Jurkat cells and staurosporine-induced cell death in corneal epithelial cells

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Standard Handling (A)

Sequence

Z-Val-Ala-Asp(OMe)-CH₂F*

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 6 months at -20°C.

Analysis Note

Single spot by TLC

Other Notes

Heng, B.C., et al. 2007. Biosci. Rep.27, 257.
Martin, U., et al. 2007. Apoptosis12, 525.
Misaghi, S., et al. 2004. Chem. Biol.11, 1677.
Joo, C., et al. 1999. Ophthalmic Res. 31, 287.
McColl, K.S., et al. 1998. Mol. Cell. Endocrinol. 139, 229.
Thornberry, N.A. and Lazebnik, Y. 1998. Science 281, 1312.
Tolomeo, M., et al. 1998. Cell Death Differ. 5, 735.
An, S. and Know, K.A. 1996. FEBS Lett.386, 115.
Cain, K., et al. 1996. Biochem J.314, 27.
Mirzoeva, O.K., et al. 1996. FEBS Lett.396, 266.
Pronk, G.J., et al. 1996. Science 271, 808.
Slee, E.A., et al. 1996. Biochem. J.315, 21.
Chow, S.C., et al. 1995. FEBS Lett. 364, 134.
Fearnhead, H.O., et al. 1995. FEBS Lett. 375, 283.
Zhu, H., et al. 1995. FEBS Lett.374, 303.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable