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627610

Sigma-Aldrich

Caspase Inhibitor I

Z-VAD-FMK, CAS 187389-52-2, is a cell-permeable, irreversible, pan-caspase inhibitor.

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Synonym(s):
Caspase Inhibitor I, Z-VAD (OMe)-FMK
Empirical Formula (Hill Notation):
C22H30FN3O7
CAS Number:
Molecular Weight:
467.49

Quality Level

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated

color

white

solubility

DMSO: 50 mM

shipped in

ambient

storage temp.

−20°C

InChI

1S/C22H30FN3O7/c1-13(2)19(26-22(31)33-12-15-8-6-5-7-9-15)21(30)24-14(3)20(29)25-16(17(27)11-23)10-18(28)32-4/h5-9,13-14,16,19H,10-12H2,1-4H3,(H,24,30)(H,25,29)(H,26,31)/t14-,16-,19-/m0/s1

InChI key

MIFGOLAMNLSLGH-QOKNQOGYSA-N

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This Item
218753218735218745
Caspase Inhibitor I Z-VAD-FMK, CAS 187389-52-2, is a cell-permeable, irreversible, pan-caspase inhibitor.

627610

Caspase Inhibitor I

Sigma-Aldrich

218753

Caspase-5 Inhibitor I

Caspase Inhibitor II The Caspase Inhibitor II, also referenced under CAS 147837-52-3, controls the biological activity of Caspase. This small molecule/inhibitor is primarily used for Cancer applications.

218735

Caspase Inhibitor II

Caspase Inhibitor III The Caspase Inhibitor III, also referenced under CAS 634911-80-1, controls the biological activity of Caspase. This small molecule/inhibitor is primarily used for Cancer applications.

218745

Caspase Inhibitor III

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

solubility

DMSO: 50 mM

solubility

DMSO: 5 mg/mL

solubility

DMSO: 150 mg/mL

solubility

DMSO: 5 mg/mL

storage condition

OK to freeze, desiccated

storage condition

OK to freeze, desiccated

storage condition

OK to freeze

storage condition

OK to freeze, desiccated

General description

A cell-permeable, irreversible, pan-caspase inhibitor. Inhibits Fas-mediated apoptosis in Jurkat cells and staurosporine-induced cell death in corneal epithelial cells. Shown to enhance the freeze-thaw survival of human embryonic stem cells. When using with purified native or recombinant enzyme, pre-treatment with an esterase is required.
A cell-permeable, irreversible, pan-caspase inhibitor. Shown to enhance the freeze-thaw survival of human embryonic stem cells. Inhibits Fas-mediated apoptosis in Jurkat T cells. Also reported to inhibit Peptide: N-glycanse (PNGase) in vitro amd in vivo. When using with a purified recombinant enzyme, pretreatment with an esterase is required.

Biochem/physiol Actions

Cell permeable: yes
Primary Target
Fas-mediated apoptosis in Jurkat cells and staurosporine-induced cell death in corneal epithelial cells
Product does not compete with ATP.
Reversible: no

Warning

Toxicity: Standard Handling (A)

Sequence

Z-Val-Ala-Asp(OMe)-CH₂F*

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 6 months at -20°C.

Analysis Note

Single spot by TLC

Other Notes

Heng, B.C., et al. 2007. Biosci. Rep.27, 257.
Martin, U., et al. 2007. Apoptosis12, 525.
Misaghi, S., et al. 2004. Chem. Biol.11, 1677.
Joo, C., et al. 1999. Ophthalmic Res. 31, 287.
McColl, K.S., et al. 1998. Mol. Cell. Endocrinol. 139, 229.
Thornberry, N.A. and Lazebnik, Y. 1998. Science 281, 1312.
Tolomeo, M., et al. 1998. Cell Death Differ. 5, 735.
An, S. and Know, K.A. 1996. FEBS Lett.386, 115.
Cain, K., et al. 1996. Biochem J.314, 27.
Mirzoeva, O.K., et al. 1996. FEBS Lett.396, 266.
Pronk, G.J., et al. 1996. Science 271, 808.
Slee, E.A., et al. 1996. Biochem. J.315, 21.
Chow, S.C., et al. 1995. FEBS Lett. 364, 134.
Fearnhead, H.O., et al. 1995. FEBS Lett. 375, 283.
Zhu, H., et al. 1995. FEBS Lett.374, 303.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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