Absorbance (1.0 M, H2O, 260 nm):≤0.1.
Widely used as a biological buffer (absorbance: <0.1 at 260 nm, 1.0 M, H2O). A 1.0 M solution in H2O has a pH range of 3.5-5.0 and a pKa of 8.3 at 20°C.
Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
250, 500 g in Glass bottle
1 kg in Glass bottle
Toxicity: Standard Handling (A)
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