648313

Tris, Hydrochloride, ULTROL^® Grade

Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C. Absorbance (1.0 M, H2O, 260 nm):≤0.1.

• Synonym(s): Trizma^® hydrochloride, tris(Hydroxymethyl)aminomethane, HCl, TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
• Linear Formula: NH₂C(CH₂OH)₃ · HCl
• CAS Number: 1185-53-1
• Molecular Weight: 157.60
• Beilstein: 3675235
• MDL number: MFCD00012590

Properties

• Quality Level: 100
• Assay: ≥99% (titration)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• useful pH range: 7.0-9.0
• pKa (25 °C): 8.1
• solubility: water: 500 mg/mL
• cation traces: heavy metals: ≤5 ppm
• shipped in: ambient
• storage temp.: 15-25°C
• SMILES string: Cl.NC(CO)(CO)CO
• InChI: 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
• InChI key: QKNYBSVHEMOAJP-UHFFFAOYSA-N

Description

General description

Absorbance (1.0 M, H₂O, 260 nm):≤0.1.

Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

Widely used as a biological buffer (absorbance: <0.1 at 260 nm, 1.0 M, H₂O). A 1.0 M solution in H₂O has a pH range of 3.5-5.0 and a pK_a of 8.3 at 20°C.

Warning

Toxicity: Standard Handling (A)

Other Notes

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable