648313

Millipore

Tris, Hydrochloride, ULTROL® Grade

Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C. Absorbance (1.0 M, H2O, 260 nm):≤0.1.

Synonym(s):
Trizma® hydrochloride, tris(Hydroxymethyl)aminomethane, HCl, TRIS HCl, Tris(hydroxymethyl)aminomethane hydrochloride, TRIS hydrochloride, Tromethane hydrochloride
Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
Molecular Weight:
157.60
Beilstein/REAXYS Number:
3675235
MDL number:

storage conditions

+15C to +30C

Quality Level

assay

≥99% (titration)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

useful pH range

7.0 - 9.0

pKa (25 °C)

8.1

solubility

water: 500 mg/mL

cation traces

heavy metals: ≤5 ppm

shipped in

ambient

storage temp.

15-25°C

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

General description

Absorbance (1.0 M, H2O, 260 nm):≤0.1.
Widely used as a biological buffer (absorbance: <0.1 at 260 nm, 1.0 M, H2O). A 1.0 M solution in H2O has a pH range of 3.5-5.0 and a pKa of 8.3 at 20°C.
Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.

Application

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Packaging

250, 500 g in Glass bottle
1 kg in Glass bottle

Warning

Toxicity: Standard Handling (A)

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Trizma is a registered trademark of Sigma-Aldrich Co. LLC
ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Protocols
This quick-start guide provides a detailed protocol for the use of the Immobilon® GO device in hands-free, western blot immunodetection.
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.