Skip to Content
MilliporeSigma
All Photos(1)

Documents

648315

Millipore

Tris Buffer, 100 mM, pH 7.4, Molecular Biology Grade

100 mM liquid, pH 7.4, Molecular Biology Grade

Sign Into View Organizational & Contract Pricing

Synonym(s):
Tris(hydroxymethyl)aminomethane, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Trometamol
Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
Beilstein/REAXYS Number:
741883
MDL number:
UNSPSC Code:
12161705
NACRES:
NA.25

grade

Molecular Biology

Quality Level

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

concentration

100 mM

color

clear colorless

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

foreign activity

DNases, RNases, none detected

shipped in

ambient

storage temp.

10-30°C

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Widely used biological buffer. Prepared with diethylpyrocarbonate-treated water for molecular biology applications. Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.
Widely used biological buffer. Suitable for DNA and RNA applications. Useful in the pH range of 7.0-9.0.

Application


  • Structural Characterization of Toxicologically Relevant Cd(2+)-L-Cysteine Complexes.: This study utilized Tris buffer, 100 mM, pH 7.4, for the structural analysis of cadmium-cysteine complexes, providing insights into their biochemical behavior and toxicological impacts (Gautam et al., 2023).

  • Cation and buffer specific effects on the DNA-lipid interaction.: The research employed Tris buffer, 100 mM, pH 7.4, to explore the specific effects of cations and buffers on DNA-lipid interactions, highlighting its importance in biophysical and biochemical studies (Mura et al., 2023).

  • Anti-inflammatory and antibacterial activities of cerium-containing mesoporous bioactive glass nanoparticles for drug-free biomedical applications.: The research utilized Tris buffer, 100 mM, pH 7.4, in the preparation of cerium-containing nanoparticles, demonstrating their potential in biomedical applications without the need for drugs (Kurtuldu et al., 2021).

Warning

Toxicity: Irritant (B)

Physical form

A sterile, 100 mM solution, pH 7.4.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 2

1 of 2

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service