648464

Triton^™ X-100, Hydrogenated, PROTEIN GRADE^® Detergent, 10% Solution, Sterile-Filtered

Non-ionic detergent used for the solubilization of membrane-bound proteins.

• Synonym(s): Octylphenoxypolyethoxyethanol, Hydrogenated
• UNSPSC Code: 12161902
• NACRES: NA.25

Properties

• description: Merck USA index - 14, 6761
• Quality Level: 100
• form: solution
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• impurities: ≤200 μM Aldehyde; ≤200 μM peroxide
• color: clear colorless
• conductivity: ≤50 μmho
• CMC: 250 μM
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Non–ionic detergent used for the solubilization of membrane-bound proteins. This purified detergent is advantageous for use with sensitive, membrane-bound proteins, overcoming the problems associated with aldehyde and peroxide impurities in polyoxyethylene detergents. Unlike its parent compound, Hydrogenated TRITON^™ X-100 Detergent lacks UV absorbance and fluorescence, which cause interference in spectophotometric monitoring and assays.

Nonionic detergent used for the solubilization of membrane-bound proteins. This purified detergent is advantageous for use with sensitive, membrane-bound proteins, overcoming the problems associated with aldehyde and peroxide impurities in polyoxyethylene detergents. Unlike its parent compound, Hydrogenated TRITON^™ X-100 lacks UV absorbance and fluorescence, which cause interference in spectophotometric monitoring and assays. Has a critical micelle concentration (CMC) of 250 µM.

Application

• Intrinsic Lipid Curvature and Bilayer Elasticity as Regulators of Channel Function: A Comparative Single-Molecule Study.: This study investigates how intrinsic lipid curvature and bilayer elasticity affect channel function using single-molecule techniques. Triton^™ X-100, Hydrogenated, PROTEIN Detergent, 10% Solution, Sterile-Filtered is used to solubilize membrane proteins, enabling detailed analysis of lipid-protein interactions and their impact on membrane dynamics (Ashrafuzzaman et al., 2024).


• Preparing for another Ebola Outbreak: The impact of viral inactivation methods on commonly measured biochemistry analytes in plasma and urine.: This study examines the effect of viral inactivation methods on biochemistry analytes in plasma and urine, using Triton^™ X-100 for viral inactivation. The findings are crucial for ensuring accurate biochemical measurements during viral outbreaks (Zhou et al., 2024).

Packaging

Packaged under inert gas

Warning

Toxicity: Harmful (C)

Physical form

10% aqueous solution.

Other Notes

Fernandez, J., et al. 1992. Anal. Biochem.201, 255.
Tiller, G.E., et al. 1984. Anal. Biochem.141, 262.
Ashani, Y. and Cantravas, G.N. 1980. Anal. Biochem.109, 55.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

PROTEIN GRADE is a registered trademark of Merck KGaA, Darmstadt, Germany

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H319,H412
• pcodes: P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Hazard Classifications: Aquatic Chronic 3 - Eye Irrit. 2 - Skin Irrit. 2
• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable