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662048

Sigma-Aldrich

Anti-Uncoupling Protein-3 (UCP-3) (147-159) Rabbit pAb

liquid, Calbiochem®

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

does not contain

preservative

species reactivity

mouse, rat

should not react with

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−70°C

General description

Recognizes the UCP-3 protein.
Protein A purified rabbit polyclonal antibody. Recognizes the UCP-3 protein.
This Anti-Uncoupling Protein-3 (UCP-3) (147-159) Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of Uncoupling Protein-3 (UCP-3) (147-159).

Immunogen

Mouse
a synthetic peptide [(C)GTGGERKYRGTMD] corresponding to amino acids 147-159 of mouse UCP-3

Packaging

100 μL in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Application

ELISA (see comments)

Immunoblotting (1:1000)

Immunocytochemistry (1:500)

Warning

Toxicity: Standard Handling (A)

Physical form

In 140 mM NaCl, 100 mM potassium phosphate, pH 7.0.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Liu, Q., et al. 1998. Gene 207, 1.
No cross reactivity with UCP1 or UCP2. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Kenji Hayata et al.
Journal of pharmacological sciences, 108(3), 348-354 (2008-11-15)
To obtain compounds that promote glucose uptake in muscle cells, the novel cell lines A31-IS derived from Balb/c 3T3 A31 and C2C12-IS from mouse myoblast C2C12 were established. In both cell lines, glucose consumption was induced by insulin and suppressed...

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