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MilliporeSigma

69670-M

His•Bind® Resin

Synonym(s):

uncharged IDA agarose beads

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10 ML

$215.00

50 ML

$855.00

100 ML

$1,390.00

$215.00

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About This Item

NACRES:
NA.56
UNSPSC Code:
41106500

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form

liquid

manufacturer/tradename

Novagen®

storage condition

do not freeze

application(s)

life science and biopharma

storage temp.

2-8°C

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application(s)

life science and biopharma

application(s)

life science and biopharma

application(s)

life science and biopharma

application(s)

-

form

liquid

form

slurry

form

liquid

form

suspension

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

-

storage condition

do not freeze

storage condition

do not freeze

storage condition

do not freeze

storage condition

-

Application

HIS-BIND® resin have been used: in purification of HIS TAG® fusion proteins by metal chelation chromatography, for purification of the bacterially produced OCRL1WT/VAR phosphatase domain to study heterogeneity in Lowe Syndrome[1], in protein purification to study anaerobic phloroglucinol degradation by Clostridium scatologenes[2], for isolation of His6-tagged proteins for studying the role of human repressor activator protein 1 (RAP1) and glial fibrillary acidic protein (GFAPɛ)proteins in increasing γ-secretase activity in a yeast model system.[3]

Biochem/physiol Actions

HIS-BIND® resin helps in the purification of proteins containing the His-tagged sequence by the process of metal chelation chromatography. It utilizes the charge buffer provided in the HIS-BIND® Buffer Kit, that binds the His-tagged sequence effectively with Ni2+ or Co2+ cations that are immobilized onto the HIS-BIND® resin. The target protein can be easily recovered by eluting with imidazole. The HIS-BIND® resin is designed to be regenerated and reused multiple times, making it a cost-effective solution. Its versatility allows for protein purification under gentle, non-denaturing conditions, or in the presence of either guanidine or urea, depending on the specific requirements of the purification process.

Disclaimer

Toxicity: Standard Handling (A)

Features and Benefits

  • Reusable many times
  • Compatible with HIS-BIND® Buffer Kit
  • Compatible with 1 mM THP

General description

His•Bind Resin is an uncharged IDA agarose resin. The binding capacity is approximately 8 mg/ml bed volume. His•Bind Resin is well suited to small to medium scale purifications using either gravity flow columns or batch methods.

Legal Information

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk

WGK 1

flash_point_f

86.0 °F

flash_point_c

30 °C

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The human RAP1 and GFAP? proteins increase ?-secretase activity in a yeast model system
Swanson MJ, et al.
G3: Genes, Genomes, Genetics, 13(8) (2023)
Heterogeneity in Lowe Syndrome: Mutations Affecting the Phosphatase Domain of OCRL1 Differ in Impact on Enzymatic Activity and Severity of Cellular Phenotypes
J Lee J, et al.
Biomolecules, 13(4), 615-615 (2023)
Anaerobic phloroglucinol degradation by Clostridium scatologenes
Zhou Y, et al.
Environmental Microbiology, 14(4) (2023)

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