69734-M

λDE3 Lysogenization Kit - Novagen

Construction of bacterial strains that carry inducible T7 RNA Polymerase

• Synonym(s): Lysogenization Kit, Novagen λDE3 Kit
• UNSPSC Code: 41106200
• NACRES: NA.51

Properties

• Quality Level: 100
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• technique(s): molecular cloning: suitable
• storage temp.: −70°C

Description

General description

Construction of bacterial strains that carry inducible T7 RNA Polymerase

The λDE3 Lysogenization Kit is designed for site-specific integration of λDE3 prophage into an E. coli host cell chromosome, such that the lysogenized host can be used to express target genes cloned in pET vectors. λDE3 is a recombinant phage carrying the cloned gene for T7 RNA polymerase under lacUV5 control. Lysogens are prepared by co-infection with the three provided phages, and can be verified by plating the T7 Tester Phage (a T7 RNA polymerase deletion mutant). This phage is unable to make a plaque on cells that lack T7 RNA polymerase, but makes normal plaques on λDE3 lysogens in the presence of IPTG.The T7 RNA Polymerase Monoclonal Antibody can be used to verify the presence of T7 RNA polymerase in the host strain by Western blot analysis.

https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-managementThis product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Components

•200 µlλDE3 Phage Lysate

•200 µlHelper Phage Lysate

•200 µlSelection Phage Lysate

•50 µlT7 Tester Phage Lysate

•200 µlHMS174(DE3) glycerol stock

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids