70188-M

pSTBlue-1 Blunt Vector (linearized) - Novagen

Properties

• form: liquid
• Quality Level: 100
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• storage temp.: −70°C

Description

General description

The pSTBlue-1 vector is a multi-purpose cloning vector featuring a versatile multiple cloning region, blue/white screening, dual opposed T7/SP6 promoters and dual kanamycin/ampicillin resistance. Restriction sites producing 4-base 3′ overhangs are conveniently positioned near each end of the polylinker to facilitate the generation of unidirectional deletions using exonuclease III.

For enhanced convenience, please consider Introductory pSTBlue-1 Perfectly Blunt^®Cloning Kit, or pT7Blue^™-1 Perfectly Blunt Cloning Kit.

Components

20 rxn40 rxn

•2 × 0.5 µg4 × 0.5 µgBlunt Vector

•10 µl10 µlPositive Control Insert

•100 µl2 × 100 µlEnd Conversion Mix

Warning

Toxicity: Standard Handling (A)

Other Notes

Novy, R.E., et al. 1996. inNovations6, 7. Clark, J.M. 1988. Nucleic Acids Res. 16, 9677. Brownstein, J.M., et al. 1996. BioTechniques20, 1004. Magnuson, V.L., et al. 1996. BioTechniques21, 700. Hu, G. 1993. DNA and Cell Biology12, 763.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

PT7BLUE is a trademark of EMD Chemicals Inc.

Perfectly Blunt is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable