70584-M

BugBuster^® Protein Extraction Reagent

Properties

• form: liquid
• Quality Level: 100
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• storage temp.: 10-30°C

Description

General description

BugBuster^® Protein Extraction Reagent is formulated for gentle disruption of Escherichia coli cell wall to liberate active proteins. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The proprietary formulation utilizes a tris-buffer-based mixture of non-ionic and zwitterionic detergents that is capable of cell wall perforation without denaturing the protein. BugBuster is also useful for the preparation of high-purity inclusion bodies for subsequent purification in instances where expressed proteins are insoluble.

Application

BugBuster^® Protein Extraction Reagent has been used:

• to lyse Escherichia coli BLR (DE3) cells for β-glucosidase (BglB) production and purification
• as a cell lysis buffer to treat the synechocystis PCC6803 transconjugants and measure the yellow fluorescent protein (YFP) fluorescence
• in lysis buffer for purification of glutathione S-transferase- heat shock protein family A (Hsp70) member 8 (GST- HSPA8)

Warning

Toxicity: Standard Handling (A)

Legal Information

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable