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Benzonase® Nuclease

Purity > 99%

Synonym(s):

Endonuclease from Serratia marcescens

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About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.54

product name

Benzonase® Nuclease, Purity > 90%,

biological source

Serratia marcescens

Quality Level

recombinant

expressed in E. coli

assay

>90% (SDS-PAGE)

form

buffered aqueous glycerol solution

manufacturer/tradename

Novagen®

storage condition

OK to freeze

concentration

25-29 units/μL

application(s)

research use

shipped in

wet ice

storage temp.

−20°C

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General description

Benzonase® Nuclease is a geneticallyengineered endonuclease from Serratiamarcescens. It degrades all forms of DNA andRNA (single stranded, double stranded, linearand circular) while having no proteolyticactivity. It is effective over a wide range ofconditions and possesses an exceptionallyhigh specific activity. The enzyme completelydigests nucleic acids to 5′-monophosphateterminated oligonucleotides 2 to 5 bases inlength (below the hybridization limit), whichis ideal for removal of nucleic acids fromrecombinant proteins, enabling compliancewith FDA guidelines for nucleic acidcontamination. The ability of Benzonase torapidly hydrolyze nucleic acids makes theenzyme an excellent choice for viscosityreduction to reduce processing time andincrease yields of protein. For example, theenzyme is compatible with BugBuster andPopCulture Protein Extraction Reagentsand can therefore be added along with thesereagents to eliminate viscosity and removenucleic acids from E. coli extracts.

The enzyme consists of two subunits of30 kDa each. It is functional betweenpH 6 and 10 and from 0-42°C and requires1-2 mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionic detergents, reducing agents, PMSF(1 mM), EDTA (1 mM) and urea (relativeactivity depends on specific conditions).Activity is inhibited by >150 mM monovalentcations, >100 mM phosphate, >100 mMammonium sulfate, or >100 mM guanidine HCl.

Benzonase Nuclease is available inultrapure (>99% by SDS-PAGE) and pure(>90%) grades at a standard concentrationof 25-29 U/µl and at a high concentration (HC)of 250 U/µl. Both preparations are free ofdetectable protease and have specific activity> 1 × 106 U/mg protein. The >99% puritygrade is tested for endotoxins and contains<0.25 EU/1000 units. The product is suppliedin 50% glycerol.Store at -20°C.

Total endotoxin:<0.25 EU/1,000 units.Purity: >90% by SDS-PAGE

Application

Used for the removal of nucleic acid from protein samples.

Biochem/physiol Actions

Digests native or heat-denatured DNA and RNA.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that causes a ΔA₂₆₀ of 1.0 in 30 minutes, which corresponds to complete digestion of 37 µg DNA.

Legal Information

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


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Mark A Kanow et al.
eLife, 6 (2017-09-14)
Here we report multiple lines of evidence for a comprehensive model of energy metabolism in the vertebrate eye. Metabolic flux, locations of key enzymes, and our finding that glucose enters mouse and zebrafish retinas mostly through photoreceptors support a conceptually
Birgitte P S Jacky et al.
PLoS pathogens, 9(5), e1003369-e1003369 (2013-05-23)
Botulinum neurotoxin serotype A (BoNT/A) causes transient muscle paralysis by entering motor nerve terminals (MNTs) where it cleaves the SNARE protein Synaptosomal-associated protein 25 (SNAP25206) to yield SNAP25197. Cleavage of SNAP25 results in blockage of synaptic vesicle fusion and inhibition
Jonathan Barroso-González et al.
Molecular cell, 76(1), 11-26 (2019-08-12)
Alternative lengthening of telomeres (ALT) is a homology-directed repair (HDR) mechanism of telomere elongation that controls proliferation in aggressive cancers. We show that the disruption of RAD51-associated protein 1 (RAD51AP1) in ALT+ cancer cells leads to generational telomere shortening. This
Chi Zhu et al.
Molecular cell, 75(4), 791-806 (2019-07-16)
YAP/TEAD are nuclear effectors of the Hippo pathway, regulating organ size and tumorigenesis largely through promoter-associated function. However, their function as enhancer regulators remains poorly understood. Through an in vivo proximity-dependent labeling (BioID) technique, we identified YAP1 and TEAD4 protein as
Tomoya Muto et al.
Nature immunology, 21(5), 535-545 (2020-04-22)
Despite evidence of chronic inflammation in myelodysplastic syndrome (MDS) and cell-intrinsic dysregulation of Toll-like receptor (TLR) signaling in MDS hematopoietic stem and progenitor cells (HSPCs), the mechanisms responsible for the competitive advantage of MDS HSPCs in an inflammatory milieu over

Articles

This page lists nine frequently asked questions and answers about Benzonase® Nuclease.

The combination of BugBuster® and Lysonase™ reagents greatly enhances the release of soluble proteins from Gram-positive bacteria.

Benzonase®endonuclease efficiently removes nucleic acid contaminants from viral production, crucial for cell and gene therapies and vaccines.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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