This product is not assigned a firm expiration date. Each lot is assigned a recommended retest date of approximately 1 year from the date of quality release. If sufficient stock remains and the material continues to meet product specifications upon reassay, the retest period is typically extended. While considered stable, the material may be subject to degradation over time. The lot-specific retest date is reported on the Certificate of Analysis.
70746
Benzonase® Nuclease
Purity > 90%
Synonym(s):
Endonuclease from Serratia marcescens
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.54
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Product Name
Benzonase® Nuclease, Purity > 90%,
biological source
Serratia marcescens
Quality Level
recombinant
expressed in E. coli
assay
>90% (SDS-PAGE)
form
buffered aqueous glycerol solution
manufacturer/tradename
Novagen®
storage condition
OK to freeze
concentration
25-29 units/μL
application(s)
research use
shipped in
wet ice
storage temp.
−20°C
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This Item | 70664 | 1.01697 | 103773 |
|---|---|---|---|
| biological source Serratia marcescens | biological source Serratia marcescens | biological source Serratia marcescens | biological source Serratia marcescens |
| assay >90% (SDS-PAGE) | assay >99% (SDS-PAGE) | assay ≥99% (SDS-PAGE) | assay ≥99% (SDS-PAGE) |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli |
| application(s) research use | application(s) research use | application(s) pharma/biopharma processes | application(s) pharma/biopharma processes |
| form buffered aqueous glycerol solution | form buffered aqueous glycerol solution | form buffered aqueous glycerol solution | form buffered aqueous glycerol solution |
| concentration 25-29 units/μL | concentration 25-29 units/μL | concentration ≥250 units/μL | concentration ≥250 units/μL |
General description
Benzonase® Nuclease is a geneticallyengineered endonuclease from Serratiamarcescens. It degrades all forms of DNA andRNA (single stranded, double stranded, linearand circular) while having no proteolyticactivity. It is effective over a wide range ofconditions and possesses an exceptionallyhigh specific activity. The enzyme completelydigests nucleic acids to 5′-monophosphateterminated oligonucleotides 2 to 5 bases inlength (below the hybridization limit), whichis ideal for removal of nucleic acids fromrecombinant proteins, enabling compliancewith FDA guidelines for nucleic acidcontamination. The ability of Benzonase torapidly hydrolyze nucleic acids makes theenzyme an excellent choice for viscosityreduction to reduce processing time andincrease yields of protein. For example, theenzyme is compatible with BugBuster andPopCulture Protein Extraction Reagentsand can therefore be added along with thesereagents to eliminate viscosity and removenucleic acids from E. coli extracts.
The enzyme consists of two subunits of30 kDa each. It is functional betweenpH 6 and 10 and from 0-42°C and requires1-2 mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionic detergents, reducing agents, PMSF(1 mM), EDTA (1 mM) and urea (relativeactivity depends on specific conditions).Activity is inhibited by >150 mM monovalentcations, >100 mM phosphate, >100 mMammonium sulfate, or >100 mM guanidine HCl.
Benzonase Nuclease is available inultrapure (>99% by SDS-PAGE) and pure(>90%) grades at a standard concentrationof 25-29 U/µl and at a high concentration (HC)of 250 U/µl. Both preparations are free ofdetectable protease and have specific activity> 1 × 106 U/mg protein. The >99% puritygrade is tested for endotoxins and contains<0.25 EU/1000 units. The product is suppliedin 50% glycerol.Store at -20°C.
Total endotoxin:<0.25 EU/1,000 units.Purity: >90% by SDS-PAGE
The enzyme consists of two subunits of30 kDa each. It is functional betweenpH 6 and 10 and from 0-42°C and requires1-2 mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionic detergents, reducing agents, PMSF(1 mM), EDTA (1 mM) and urea (relativeactivity depends on specific conditions).Activity is inhibited by >150 mM monovalentcations, >100 mM phosphate, >100 mMammonium sulfate, or >100 mM guanidine HCl.
Benzonase Nuclease is available inultrapure (>99% by SDS-PAGE) and pure(>90%) grades at a standard concentrationof 25-29 U/µl and at a high concentration (HC)of 250 U/µl. Both preparations are free ofdetectable protease and have specific activity> 1 × 106 U/mg protein. The >99% puritygrade is tested for endotoxins and contains<0.25 EU/1000 units. The product is suppliedin 50% glycerol.Store at -20°C.
Total endotoxin:<0.25 EU/1,000 units.Purity: >90% by SDS-PAGE
Application
Used for the removal of nucleic acid from protein samples.
Biochem/physiol Actions
Digests native or heat-denatured DNA and RNA.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that causes a ΔA₂₆₀ of 1.0 in 30 minutes, which corresponds to complete digestion of 37 µg DNA.
Legal Information
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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How long can the product be stored at -20°C?
1 answer-
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How can 70746 Benzofane be inactivated without using NaOH?
1 answer-
Benzonase can be inactivated through various methods such as treatment with NaOH, EDTA and warming with EDTA at 70°C, or guanidine HCl. However, it is important to note that a guanidine HCl concentration exceeding 100 mM can completely inhibit the enzyme activity. Additionally, while an EDTA concentration of 1mM partially inhibits Benzonase endonuclease, a concentration of 5 mM EDTA leads to a loss of over 90% of enzyme activity due to the complexing of the essential Mg2+ ions.
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Can proteinase K be utilized to inactivate Benzonase? This is to prevent further DNA degradation post-sampling. Would it be adequate to treat it with a final concentration of 10 mg/ml of proteinase K for 20 minutes at 55°C?
1 answer-
There is currently no available data on the conditions required for inactivating benzonase by proteinase K. However, it is known that benzonase can be reversibly inactivated by metal ion chelators, such as EDTA and EGTA. Irreversible inactivation can also be achieved through extreme conditions (100 mM NaOH at 70°C for 30 minutes).
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