70856

RNase A Solution

Convenient solution for selective degradation of RNA

• Synonym(s): RNase A Enzyme
• UNSPSC Code: 12352202
• NACRES: NA.54

Properties

• form: solution
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze
• concentration: 10 mg/mL
• technique(s): DNA purification: suitable
• shipped in: ambient
• storage temp.: 10-30°C

Description

General description

RNase A Solution is a convenient alternative to powdered RNase A. It is a highly purified preparation of bovine pancreatic ribonuclease A suitable for use in selective removal of RNA. It has been pretreated to remove DNase I and is suitable to remove RNA contamination during plasmid and genomic DNA[1] purification procedures. Supplied at a concentration of 10 mg/ml in 10 mM Tris-HCl, 1 mM EDTA, 50% glycerol, pH 7.5.

Application

RNase A Solution has been used:

• in cell cycle profiling of embryonic mouse neuroectodermal cells (NE-4C)[2]
• in the digestion of RNA for extraction of DNA from human osteosarcoma cell lines for chromatin immunoprecipitation (ChIP) assays[3]
• to treat control adult and embryonic thymi cells during staining[4]
• to remove RNA from affibody–mRNA fusion clones before clone screening[5]

Biochem/physiol Actions

RNaseA is an endoribonuclease that catalyzes the degradation of phosphodiester linkages with a pyrimidine base at the 3′ -position on single-stranded RNA via, transphosphorylation and hydrolysis mechanism.[6]

Warning

Toxicity: Standard Handling (A)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2