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70955

Millipore

5% Alkali-soluble Casein

Unique blocking reagent for ultra-low background

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Synonym(s):
blocking buffer, immunodetection blocking buffer

form

liquid

manufacturer/tradename

Novagen®

storage condition

OK to freeze

shipped in

ambient

storage temp.

2-8°C

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This Item
C37771.46680C7278
Millipore

Millipore

70955

5% Alkali-soluble Casein

Casein fluorescein isothiocyanate from bovine milk Type II, essentially salt-free, lyophilized powder

Sigma-Aldrich

C3777

Casein fluorescein isothiocyanate from bovine milk

Millipore

Millipore

1.46680

Casein Broth + LT

Caseinoglycopeptide from bovine casein essentially salt-free, lyophilized powder

Sigma-Aldrich

C7278

Caseinoglycopeptide from bovine casein

manufacturer/tradename

Novagen®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

storage condition

OK to freeze

storage condition

-

storage condition

-

storage condition

-

shipped in

ambient

shipped in

-

shipped in

-

shipped in

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-25°C

storage temp.

2-8°C

General description

Alkali-Soluble Casein is a superior blocking reagent for Western blot applications. This preparation minimizes non-specific background relative to other blocking reagents, such as BSA, gelatin, or non-fat milk. Alkali-Soluble Casein is effective in colorimetric and chemiluminescent detection protocols. Note that due to its high coating properties, detection sensitivity for some antibodies may be lower than with other blocking reagents under the same conditions (although the background is also reduced); the concentration can be optimized for maximal signal:noise ratios. Supplied as a 5% w/v solution; recommended working concentration range is 0.251%.
Unique blocking reagent for ultra-low background

Warning

Toxicity: Standard Handling (A)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317 - H412

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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T1503
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25G
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Sigma-Aldrich

C3400

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Protocols

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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