Alkali-Soluble Casein is a superior blocking reagent for Western blot applications. This preparation minimizes non-specific background relative to other blocking reagents, such as BSA, gelatin, or non-fat milk. Alkali-Soluble Casein is effective in colorimetric and chemiluminescent detection protocols. Note that due to its high coating properties, detection sensitivity for some antibodies may be lower than with other blocking reagents under the same conditions (although the background is also reduced); the concentration can be optimized for maximal signal:noise ratios. Supplied as a 5% w/v solution; recommended working concentration range is 0.251%.
Unique blocking reagent for ultra-low background