71086
KOD Hot Start DNA Polymerase
High fidelity DNA polymerase designed for accurate PCR amplification of long strand and GC- rich DNA templates for cloning and cDNA amplification applications.
Synonym(s):
High fidelity PCR, Hot start PCR, Hot start polymerase, KOD hot start polymerase
About This Item
Recommended Products
recombinant
expressed in E. coli
Quality Level
100
200
usage
sufficient for 1000 reactions
sufficient for 200 reactions
feature
Difficult Templates/Specialty Enzymes PCR
High Fidelity PCR
dNTPs included
hotstart
manufacturer/tradename
Novagen®
storage condition
OK to freeze
concentration
1.0 U/L
technique(s)
PCR: suitable
input
purified DNA
suitability
suitable for PCR
shipped in
dry ice
storage temp.
−20°C
General description
Source: Recombinant Thermococcus kodakaraensis KOD1 DNA polymerase expressed in E. coli
Concentration: 1.0 U/l
Nicking activity: None detected
Amplification effiency: Functional PCR; inhibition of activity at 21°C verified
Storage: -20°C
*Manufactured by Toyobo and distributed by EMD. Not available in Japan.
Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser¢s own internal research. No other patents rights (such as 5¢ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
Application
- in the amplification of genomic DNA with high fidelity for sanger sequencing.
- for genomic DNA amplification to screen single-cell clones for homozygous deletion of the targeted exon.
- for the amplification of specific gene families on the genomic DNA isolated from mice lymphoma cells.
Features and Benefits
- High Fidelity: Approximately 80-fold higher fidelity than conventional Taq DNA polymerase
- Long & Accurate PCR: Amplifies genomic DNA up to 12 kbp and plasmid and lambda DNA templates up to 21 kbp with low error rate
- Efficiently amplifies high GC-rich regions
- Antibody-mediated hot start feature reduces mispriming and primer-dimer formation
- Produces blunt end PCR products suitable for cloning
- Convenient room-temperature setup compatible with automation
- Compatible with site-directed mutagenesis protocols
- Optimal KOD Hot Start Buffer for PCR performance over a wide range of targets
Components
•1.2 ml or 5 × 1.2 ml10X PCR Buffer for KOD Hot Start DNA Polymerase
•1 ml or 5 × 1 ml25 mM MgSO₄
•1 ml or 5 × 1 mldNTP Mix (2 mM each)
Warning
Unit Definition
Other Notes
Legal Information
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
Certificates of Analysis (COA)
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The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.
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