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MilliporeSigma

71259

Sigma-Aldrich

Insect GeneJuice® Transfection Reagent

Proprietary liposome transfection reagent optimized for maximal transfection efficiency of Sf9 insect cells for baculovirus protein expression.

Synonym(s):

GeneJuice Transfection

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About This Item

UNSPSC Code:
41106502
NACRES:
NA.54

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Quality Level

100

form

suspension

manufacturer/tradename

Novagen®

storage condition

OK to freeze

technique(s)

transfection: suitable

shipped in

wet ice

storage temp.

2-8°C

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709677128171115
Insect GeneJuice® Transfection Reagent Proprietary liposome transfection reagent optimized for maximal transfection efficiency of Sf9 insect cells for baculovirus protein expression.

Sigma-Aldrich

71259

Insect GeneJuice® Transfection Reagent

GeneJuice® Transfection Reagent Non-lipid based chemical transfection reagent optimized for maximum transfection efficiency, ease-of-use, and minimal cytotoxicity on a wide variety of mammalian cells.

Sigma-Aldrich

70967

GeneJuice® Transfection Reagent

ProteoJuice Protein Transfection Reagent Efficient protein delivery into mammalian cells

Sigma-Aldrich

71281

ProteoJuice Protein Transfection Reagent

RiboJuice siRNA Transfection Reagent Efficient delivery of siRNA for targeted gene suppression

Sigma-Aldrich

71115

RiboJuice siRNA Transfection Reagent

form

suspension

form

liquid

form

liquid

form

liquid

technique(s)

transfection: suitable

technique(s)

transfection: suitable

technique(s)

transfection: suitable

technique(s)

transfection: suitable

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

storage condition

OK to freeze

storage condition

OK to freeze

storage condition

OK to freeze

storage condition

do not freeze

General description

Insect GeneJuice<TMSYMBOL></TMSYMBOL> Transfection Reagent is a proprietary liposome formulation optimized for maximal transfection efficiency of Sf9 insect cells. The reagent features extremely low toxicity to the cells and can be used for both transient and stable transfections in serum-containing or serum-free media, and for cotransfection of transfer plasmids with linearized virus DNA for the production of recombinant baculoviruses. Insect GeneJuice is ideal for large-scale protein expression in suspension-culture transfections of Sf9 insect cells when using pIEx<TMSYMBOL></TMSYMBOL> and pBiEx<TMSYMBOL></TMSYMBOL> vectors, which contain the immediate early baculovirus promoter, IE1.

  • When Insect Genejuice® used in conjunction with Sf9 cell line, raw relative light units (RLUs) from Renilla Luciferase expression with plex is 131,90 Vs. 75,726 when other reagent used with Sf9, thus 1.7 fold difference.
  • When Insect Genejuice® used in conjunction with Sf9 cell line, raw relative light units (RLUs) from Renilla Luciferase expression with plex is 131,90 Vs. 11,952 when other reagent used with High Five, thus 11 fold difference.
  • When Insect Genejuice® used in conjunction with Sf9 cell line, raw relative light units (RLUs) from Renilla Luciferase expression with plex is 131,90 Vs. 31,897 when Insect Genejuice used with High Five, thus 4.1 fold difference.

Insect GeneJuice Transfection Reagent is provided as a 2 mg/ml suspension in 20 mM MES, 150 mM NaCl, pH 6.2 buffer. One ml is sufficient for 12 or 125 transfections in 10-ml suspension culture flasks or 35-mm plates, respectively.

Legal Information

GeneJuice is a registered trademark of EMD Biosciences, Inc., Madison, WI USA
GENEJUICE is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Insect GeneJuice Transfection Reagent
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