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71296

Millipore

PhosphoSafe Extraction Reagent

Novagen′s PhosphoSafe Extraction Buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state.

Synonym(s):
Protein Extraction Reagent

Quality Level

form

liquid

manufacturer/tradename

Novagen®

storage condition

OK to freeze

shipped in

dry ice

storage temp.

−70°C

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Pyrophosphate reagent

manufacturer/tradename

Novagen®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

storage condition

-

storage condition

-

storage condition

OK to freeze, protect from light

shipped in

dry ice

shipped in

wet ice

shipped in

-

shipped in

ambient

storage temp.

−70°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

General description





PhosphoSafe Extraction Buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state. This reagent contains the same formula as CytoBuster Protein Extraction Reagent (Trim 2001), but also includes four phosphatase inhibitors: sodium fluoride, sodium vanadate, β-glycerophosphate, and sodium pyrophosphate. PhosphoSafe Extraction Reagent is compatible with kinase assays, protein interaction analysis, and other applications. This reagent is provided in convenient 25-ml bottles (1 bottle for 71296-3, 5 bottles for 71296-4).

Features and Benefits

  • Phosphorylation state preserved during extraction
  • Compatible with kinase assays

Warning

Toxicity: Toxic (F)

Other Notes

Trim, J.E. and Sawyer, D.L. 2001. inNovations12, 5.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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25G
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