This product is in solution and comes ready for transformation.
71341
pRSFDuet-1 DNA - Novagen
Synonym(s):
pRSFDuet-1 Vector
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10 μG
$589.00
$589.00
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About This Item
UNSPSC Code:
41106602
NACRES:
NA.51
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form
liquid
Quality Level
manufacturer/tradename
Novagen®
storage condition
OK to freeze
promoter
Promoter name: T7
selection
kanamycin
shipped in
wet ice
storage temp.
−70°C
1 of 4
This Item | 71129 | 70608 | 71147 |
|---|---|---|---|
| promoter Promoter name: T7 | promoter - | promoter - | promoter Promoter name: T7 |
| form liquid | form liquid | form liquid | form liquid |
| storage temp. −70°C | storage temp. −70°C | storage temp. −20°C | storage temp. −70°C |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename Novagen® |
| storage condition OK to freeze | storage condition OK to freeze | storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition OK to freeze |
General description
pRSFDuet-1 is designed for the co-expression of two target proteins. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (RBS). The vector also carries the pRSF1030 replicon (also known as NTP1), LacI gene, and kanamycin resistance marker.
Application
pRSFDuet-1 DNA - Novagen has been used as a vector:
- to clone the DNA fragments of defensin α 5 (DEFA5) for recombinant expression[1]
- to clone the coding region of Mad1597–719[2]
- to clone the globular domain of Bombyx mori Spc24 (73-162aa) with an N-terminal 6X His-tag to generate the B. mori 6xHis-Spc24(73-162)-Spc25(70-211) pRSF-DUET1 bacterial expression constructs[3]
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Qiao Qiao et al.
Experimental cell research, 407(2), 112809-112809 (2021-09-07)
Defensins are highly conserved antimicrobial peptides, which ubiquitously expressed in different species. In addition to the functions in host defense, their aberrant expression have also been documented in cancerous tissue including breast cancer, lung caner and renal carcinoma etc. Whereas
Elyse S Fischer et al.
EMBO reports, 22(7), e52242-e52242 (2021-05-21)
During metaphase, in response to improper kinetochore-microtubule attachments, the spindle assembly checkpoint (SAC) activates the mitotic checkpoint complex (MCC), an inhibitor of the anaphase-promoting complex/cyclosome (APC/C). This process is orchestrated by the kinase Mps1, which initiates the assembly of the
Mohannad Al Chiblak et al.
BMC molecular and cell biology, 20(1), 60-60 (2019-12-21)
ZNF746 and ZNF777 belong to a subset of the large Krüppel-associated box (KRAB) zinc finger (ZNF) transcription factor family. They contain, like four other members in human, an additional conserved domain, the "domain of unknown function 3669" (DUF3669). Previous work
Nuria Cortes-Silva et al.
Current biology : CB, 30(4), 561-572 (2020-02-08)
Accurate chromosome segregation requires assembly of the multiprotein kinetochore complex at centromeres. In most eukaryotes, kinetochore assembly is primed by the histone H3 variant CenH3 (also called CENP-A), which physically interacts with components of the inner kinetochore constitutive centromere-associated network
Related Content
With our protein expression platforms, you can choose the proteins you purify to answer the precise biological questions you’re tackling. Don’t let limitations like solubility, yield, or toxicity influence your choice of proteins to study. With the expertise of Novagen®, the household name in cloning and recombinant protein expression technology, we make it possible to elucidate meaningful functions of even the most intractable gene products.
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Hi, does this plasmid come ready for transformation into cells or does it come lyophilized? Kind regards John
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