Yes, the Origami 2 host strains have deletion mutations in both the thioredoxin reductase (trxB) and glutathione reductase (gor) genes resulting in a natural expression. These are permanant deletions which do not require the use of antibiotics to maintain expression. Please see the link below to review additional information:
https://www.emdmillipore.com/product/Origami-2DE3pLysS-Competent-Cells-Novagen,EMD_BIO-71346#anchor_USP
71346-M
Origami 2(DE3)pLysS Competent Cells - Novagen
Escherichia coli, rod shaped
Synonym(s):
Novagen Competent Cells
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 0.4 mL | Check Cart for Availability | $181.00 | |
| 1 mL | Check Cart for Availability | $361.00 |
About This Item
UNSPSC Code:
41106202
NACRES:
NA.81
Biological source:
Escherichia coli
Growth mode:
adherent or suspension
Morphology:
rod shaped
Manufacturer/tradename:
Novagen®
$181.00
Check Cart for Availability
Product Name
Origami 2(DE3)pLysS Competent Cells - Novagen, Origami 2 strains have mutations in glutathione reducatase(gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation. T7 lysozyme expression suppresses basal T7 expression.
biological source
Escherichia coli
Quality Level
manufacturer/tradename
Novagen®
storage condition
OK to freeze
growth mode
adherent or suspension
morphology
rod shaped
technique(s)
microbiological culture: suitable
cell transformation
transformation efficiency: 2×106 cfu/μg
shipped in
dry ice
storage temp.
−70°C
General description
Genotype: Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139ahpC galE galK rpsL F′[lac+ lacIq pro] (DE3) gor522::Tn10trxB pLysS (CamR, StrR, TetR)
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Origami 2 strains have mutations in glutathione reducatase(gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation. T7 lysozyme expression suppresses basal T7 expression.
Origami 2 host strains are K-12derivatives that have mutations in both thethioredoxin reductase (trxB) and glutathionereductase (gor) genes, which greatly enhancedisulfide bond formation in the E. colicytoplasm. The Origami2 strains are kanamycin sensitive; like theoriginal Origami strains, the gor mutation is still selectedfor by tetracycline. To reduce the possibility ofdisulfide bond formation between molecules,strains containing mutations in trxB and gor arerecommended only for the expression of proteinsthat require disulfide bond formation for properfolding.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
Other Notes
0.4 ml1 mlComponent
•2 × 0.2 ml5 × 0.2 mlOrigami 2(DE3)pLysS Competent Cells
•2 × 2 ml4 × 2 mlSOC Medium
•2 ng2 ngTest Plasmid
•2 × 0.2 ml5 × 0.2 mlOrigami 2(DE3)pLysS Competent Cells
•2 × 2 ml4 × 2 mlSOC Medium
•2 ng2 ngTest Plasmid
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
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This Item | |||
|---|---|---|---|
| technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) - |
| biological source Escherichia coli | biological source Escherichia coli | biological source Escherichia coli | biological source - |
| growth mode adherent or suspension | growth mode adherent or suspension | growth mode adherent or suspension | growth mode - |
| morphology rod shaped | morphology rod shaped | morphology rod shaped | morphology - |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| storage temp. −70°C | storage temp. −70°C | storage temp. −70°C | storage temp. −70°C |
Storage Class
10 - Combustible liquids
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Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 71346-4 | 04055977253344 |
| 71346-3 | 04055977253337 |
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Is the trxB gene naturally expressed in Origami 2 cells? If it is not naturally expressed, what antibiotics are required for expression?
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