71456

BugBuster^® Master Mix

• Synonym(s): BugBuster^® Protein Extraction Reagent with Benzonase^® Nuclease and rLysozyme solution, Cell Lysis Master Mix
• UNSPSC Code: 41106511
• NACRES: NA.32

Properties

• form: liquid
• Quality Level: 200
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

The BugBuster^® Master Mix enables efficient protein purification and other related applications. It replaces the need for mechanical methods like the French press or sonication. The two available package sizes contain sufficient reagents for extracting proteins from either 20 grams or 100 grams of cell paste.

Application

BugBuster^® Master Mix has been used:

• to resuspend and lyse the cell pellets during protein purification[1]
• to extract proteins for immunoblotting[2]
• as a bacterial lysis buffer for the preparation of inclusion bodies[3]

    

Biochem/physiol Actions

BugBuster^® Protein Extraction Reagent is specifically designed to gently disrupt the cell wall of E. coli, allowing the release of soluble proteins. This unique formulation utilizes a detergent mix that can effectively perforate the cell wall without denaturing the soluble proteins. By combining BugBuster Reagent, rLysozyme solution, and Benzonase Nuclease, our BugBuster Master Mix enhances protein extraction efficiency and simplifies downstream processing of protein extracts.

Features and Benefits

• Efficient lysis of Gram-positive and Gram-negative bacteria
• Maximum recovery of active, soluble protein
• Capable of cell wall perforation without denaturing soluble protein
• Useful for the preparation of high purity inclusion bodies
• Compatible with protease and phosphatase inhibitor cocktail

• Offers a convenient, fast, and cost-effective solution for releasing the expressed target protein.
• The Master Mix eliminates the need to dilute or perform separate addition steps.

Warning

Toxicity: Standard Handling (A)

Legal Information

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable