71491

Overnight Express^™ Instant TB Medium - Novagen

• Synonym(s): autoinduction media
• UNSPSC Code: 41106213
• NACRES: NA.54

Properties

• form: solid
• Quality Level: 200
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze
• technique(s): microbiological culture: suitable
• application(s): microbiology
• shipped in: ambient
• storage temp.: 10-30°C

Description

General description

Overnight Express^™ Instant TB Medium is a complete autoinduction media in granulated terrific broth formulation. It is used in routine recombinant protein expression applications. Overnight Express Instant TB Medium is ideal for producing recombinant protein without monitoring cell growth or inducer (Isopropyl ß-D-1-thiogalactopyranoside(IPTG)) addition through lac operon-controlled Escherichia coli expression systems.

Application

Overnight Express Instant TB Medium – Novagen has been used:

• to culture recombinant Escherichia coli colonies for protein expression.[1][2][3]
• as an auto-induction media to study the effect of glucose and lactose additives on recombinant ovine angiotensinogen (oANG) production[4]
• as a medium for hamster recombinant PrP (rPrP) protein expression[5]

Features and Benefits

• Autoinduction media: simplifies protocol by eliminating the monitoring and induction steps
• Rich culture media: high cell densities and protein expression level
• Granulated format: rapid and uniform dissolution in water minimize the chance of inhaling hazardous powdered ingredients
• Singe use EasyPak packaging: Minimal sample handling significantly reduces the chance of contamination.
• Convenient, no need to spend extra time on weighting out different ingredients

Warning

Toxicity: Standard Handling (A)

Preparation Note

The EasyPak aluminum foil pouch contains enough granulated medium to prepare 1 L culture. Just add the EasyPak contents to 1 L sterile water, supplement with 10 ml glycerol, and bring it to a boil in a microwave oven for 2 minutes.

Legal Information

Express is a trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3