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71493-M

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HRV 3C Protease

Highly efficient fusion tag removal at low temperature

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Quality Level

manufacturer/tradename

Novagen®

storage condition

OK to freeze

concentration

1.8-3.0 unit/μL

shipped in

dry ice
wet ice

storage temp.

−20°C

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This Item
SAE0110SAE0045SAB5700598
Millipore

Millipore

71493-M

HRV 3C Protease

HRV-3C Protease. N-Terminal His tagged recombinant protein, aqueous solution, 0.8-1.2 mg/mL

Sigma-Aldrich

SAE0045

HRV-3C Protease.

storage condition

OK to freeze

storage condition

-

storage condition

-

storage condition

-

concentration

1.8-3.0 unit/μL

concentration

0.8-1.2 mg/mL

concentration

0.8-1.2 mg/mL

concentration

-

shipped in

dry ice, wet ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

Quality Level

400

Quality Level

-

Quality Level

200

Quality Level

100

General description

Recombinant type 14 3C protease from human rhinovirus (HRV 3C) is a recombinant restriction-grade protease. HRV 3C is a highly purified recombinant 6XHis-fusion protein, that recognizes the same cleavage site as the native enzyme: LeuGluValLeuPheGln?GlyPro. The small, 22-kDa size of the protease, optimal activity at 4°C, high specificity, and His Tag® fusion make HRV 3C protease an ideal choice for rapid removal of fusion tags. The pET expression vectors pET-47b(+) through pET-50b(+) incorporate the HRV 3C protease cleavage site in combinations withHis Tag®, S•Tag, thioredoxin (Trx•Tag), glutathione-S-transferase (GST•Tag), and NusA (Nus•Tag) sequences. The combination of pET-47b(+) to pET-50b(+) vectors for expression, HRV 3C protease for fusion tag cleavage, and Ni-NTA His•Bind metal affinity chromatography for protein purification as well as protease and fusion tag removal allows production of recombinant proteins free of vector-encoded sequences. Other expression vectors, including the pET-52b(+) vector, the InsectDirect pIEx-9 vector, and the multisystem pTriEx-6 vector, all incorporate the HRV 3C protease cleavage site in combination with the Strep•Tag II and His•Tag sequences. Supplied as: 1.8-3.0 U/µl in 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 0.5 mM THP, and 50% glycerol.

Application

  • One unit will cleave >95% of 100 µg His•Tag fusion control protein in 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 at 4°C for 16 h.

Components

  • 500 U - HRV 3C Protease
  • 10 µg - HRV 3C Cleavage Control Protein
  • 10 ml - 10X HRV 3C Cleavage Buffer

Warning

Toxicity: Standard Handling

Physical form

In 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 0.5 mM THP, and 50% glycerol.

Legal Information

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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