The pTriEx<TMSYMBOL></TMSYMBOL>-6 vector is uniquely designed to allow rapid characterization of target genes in multiple expression systems. With this vector, a single recombinant plasmid can be used to test expression in E. coli, insect and mammalian cells. Transient mammalian expression is mediated by the CMV immediate early enhancer and promoter. For expression in insect cells, pTriEx-6 contains flanking baculovirus sequences to permit the generation of recombinant baculoviruses using the BacVector<TMSYMBOL></TMSYMBOL> System or BacMagic<TMSYMBOL></TMSYMBOL> Kits. In baculovirus-infected insect cells, expression is driven by the very late p10 promoter. Expression in E. coli is regulated by the tightly controlled T7lac promoter. For expression in E. coli, pTriEx recombinant plasmids can be transferred into a (DE3)pLacI host that allows IPTG based induction. The pTriEx-6 vector contains an N-terminal 8 aa Strep•Tag<TMSYMBOL></TMSYMBOL> II coding sequence followed by a HRV 3C protease cleavage site and a multiple cloning site, with the option to include a C-terminal thrombin protease cleavage site and His•Tag<TMSYMBOL></TMSYMBOL> coding sequence with 10 histidines. pTriEx-6 is also available as a 3C/LIC Vector Kit.
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