71842
KOD Hot Start Master Mix
ready-to-use solution, Ready-to-use 2X mixture, containing KOD Hot Start DNA Polymerase, two monoclonal antibodies, ultrapure deoxynucleotides, and reaction buffer with MgSO4, optimized for convenient high fidelity PCR., suitable for PCR
Synonym(s):
High fidelity master mix, Hot start PCR, Hot start master mix
About This Item
Recommended Products
Quality Level
form
ready-to-use solution
usage
sufficient for 100 reactions
sufficient for 500 reactions
feature
Difficult Templates/Specialty Enzymes PCR
High Fidelity PCR
dNTPs included
hotstart
manufacturer/tradename
Novagen®
storage condition
OK to freeze
technique(s)
PCR: suitable (2X PCR Master Mix for high fidelity)
PCR: suitable
input
purified DNA
shipped in
dry ice
storage temp.
−20°C
Related Categories
General description
Application
- To yield PCR product for T7 endonuclease I (T7EI) assay.
- For PCR amplification of the DNA isolated from Chrysosporium multifidum, a fungus with antimicrobial activity.
- In the PCR amplification of cDNA.
- In two-step PCR for the amplification of the target sequence followed by its insertion into a vector.
Features and Benefits
- Highest accuracy, yield, and processivity of commercially available proofreading DNA polymerases
- Amplifies genomic DNA templates up to 12 kbp
- Amplifies plasmid and lambda DNA template up to 21 kbp
- Eliminates mispriming and primer-dimer formation
- Convenient ambient-temperature setup compatible with automation
- KOD HotStart® Buffer ensures optimal PCR performance over a wide range of targets.
- Offers high specificity, fidelity and yield during PCR amplification
- Optimized for ease of use and data reproducibility
- Simplifies PCR set-up and saves time
- High consistency with minimal risk of contamination
*Manufactured by Toyobo and distributed by Merck KGaA, Darmstadt, Germany and/or its affiliates (not in Japan).
Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patents rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
Components
- 2 × 1.25 ml or 10 × 1.25 mL KOD Hot Start Master Mix
References:
Kitabayashi, M., et al. 2002. Biosci. Biotechnol. Biochem.66 (10), 2194. Fujii, S., et al. 1999. J. Mol.
Warning
Unit Definition
Other Notes
Legal Information
Related product
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Articles
PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.
The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.
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Efficiently amplify your samples with confidence using KOD One™ PCR Master Mix for ultra-fast PCR with one of the fastest elongation rates available for high fidelity polymerases.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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