8.51025

5-Carboxyfluorescein

Novabiochem^®

• Synonym(s): 5-Carboxyfluorescein, 5-FAM
• Empirical Formula (Hill Notation): C₂₁H₁₂O₇
• CAS Number: 76823-03-5
• Molecular Weight: 376.32
• UNSPSC Code: 12352005
• NACRES: NA.22
• MDL number: MFCD00036874

Properties

• Quality Level: 200
• product line: Novabiochem^®
• assay: ≥98% (TLC), ≥99.0% (HPLC)
• form: powder
• reaction suitability: reagent type: fluorescent-labelling reagent
• manufacturer/tradename: Novabiochem^®
• application(s): peptide synthesis
• storage temp.: 2-8°C
• SMILES string: O1C3(c4c(cc(cc4)O)Oc5c3ccc(c5)O)c2c(cc(cc2)C(=O)O)C1=O
• InChI: 1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)
• InChI key: NJYVEMPWNAYQQN-UHFFFAOYSA-N

Description

General description

This high purity fluorescent dye (λ_ex494 nm, λ_em 518 nm) is supplied as a single isomer, which ensures production of labeled products of defined chemical structure, as well as greatly simplifying purification and characterization. FAM should be introduced as the last step in the synthesis to avoid formation of side-products, or be protected by tritylation prior to acylation or hydrazine treatment ^[1].

Associated Protocols and Technical Articles
Chromogenic Labeling of Synthetic Peptides

Literature references

^[1] R. Fischer, et al. (2003) Bioconjugate Chem., 14, 653.

Application

5-Carboxyfluorescein Novabiochem^®. CAS 76823-03-5, molar mass 376.32 g/mol.

Analysis Note

Colour (visual): yellow to orange
Appearance of substance (visual): powder
Identity (IR): passes test
Purity (TLC (EtOAc/MeOH/H2O/DIPEA 60 : 30 : 10 : 3)): ≥ 98 %
Assay (HPLC, area%): ≥ 99.0 % (a/a)

To see the solvent systems used for TLC of Novabiochem^® products please click here.

Other Notes

Replaces: 01-63-0112

Legal Information

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable