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8.51029

Sigma-Aldrich

N-Biotinyl-NH-(PEG)₂-COOH . DIPEA (20 atoms)

Novabiochem®

Synonym(s):

N-Biotinyl-NH-(PEG)₂-COOH . DIPEA (20 atoms), O-(N-Biotinyl-3-aminopropyl)-O′-(N-glutaryl-3-aminopropyl)-diethyleneglycol · DIPEA

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500 MG
$445.00
1 G
$676.80

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Estimated to ship onApril 11, 2025


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500 MG
$445.00
1 G
$676.80

About This Item

Empirical Formula (Hill Notation):
C25H44N4O8S · xC8H19N
CAS Number:
Molecular Weight:
560.70
MDL number:
UNSPSC Code:
12352106
NACRES:
NA.22

$445.00


Estimated to ship onApril 11, 2025


Request a Bulk Order

Quality Level

product line

Novabiochem®

assay

≥95.0% (HPLC)
≥98% (TLC)

form

powder

reaction suitability

reaction type: Biotinylations

manufacturer/tradename

Novabiochem®

mp

131-136 °C

application(s)

peptide synthesis

storage temp.

2-8°C

SMILES string

S1[C@H]([C@H]2NC(=O)N[C@H]2C1)CCCCC(=O)NCCOCCOCCOC(=O)O.N(C(C)C)(C(C)C)CC

InChI

1S/C17H29N3O7S.C8H19N/c21-14(18-5-6-25-7-8-26-9-10-27-17(23)24)4-2-1-3-13-15-12(11-28-13)19-16(22)20-15;1-6-9(7(2)3)8(4)5/h12-13,15H,1-11H2,(H,18,21)(H,23,24)(H2,19,20,22);7-8H,6H2,1-5H3/t12-,13-,15-;/m0./s1

InChI key

YOALVTBRBBZXGM-HZPCBCDKSA-N

General description

The use of this novel biotin derivative incorporates a hydrophilic 20 atom PEG spacer between the peptide and biotin. This not only greatly improves the solubility of the resultant peptide, but reduces steric hindrance between the peptide and biotin, leading to better avidin binding and higher biological activity. The biotin-PEG derivative can be easily incorporated using any standard coupling method as the final step in SPPS, immediately prior to cleavage of the peptide from the resin.

Associated Protocols and Technical Articles

Biotinylation Reagents for Peptide Synthesis

Linkage

Replaces: 01-63-0133

Analysis Note

Color (visual): white to slight yellow to beige
Appearance of substance (visual): powder
Identity (IR): passes test
Purity (TLC(CMA2)): ≥ 98 %
Assay (HPLC, area%): ≥ 95.0 % (a/a)
Solubility (50 mg in 1 ml NMP): clearly soluble

To see the solvent systems used for TLC of Novabiochem® products please click here.

Legal Information

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Articles

Biotin-labelled peptides find applications in immunology and histochemistry for affinity purification and receptor localization.

Questions

  1. The molecular weight say 560, but I believe you are missing the DIPEA. What is the true MW of this compound?

    1 answer
    1. Thank you for calling this to our attention. The molecular weight of this product is 698.95 g/mol. This value will be corrected shortly.
      Please see the link below to review which offers the correct molecular weight:
      https://www.emdmillipore.com/product/N-Biotinyl-NH-PEG2-COOH-.-DIPEA-20-atoms,MDA_CHEM-851029

      Helpful?

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