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8.51082

Sigma-Aldrich

5(6)-Carboxyfluorescein

Novabiochem®

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Synonym(s):
5(6)-Carboxyfluorescein
Empirical Formula (Hill Notation):
C21H12O7
CAS Number:
Molecular Weight:
376.32
MDL number:
EC Index Number:
276-331-1

Quality Level

product line

Novabiochem®

form

powder

reaction suitability

reagent type: fluorescent-labelling reagent

manufacturer/tradename

Novabiochem®

application(s)

peptide synthesis

storage temp.

2-8°C

InChI

1S/2C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21;22-11-2-5-14-17(8-11)27-18-9-12(23)3-6-15(18)21(14)16-7-10(19(24)25)1-4-13(16)20(26)28-21/h2*1-9,22-23H,(H,24,25)

InChI key

BPVHBBXCESDRKW-UHFFFAOYSA-N

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5(6)-Carboxyfluorescein Novabiochem®

8.51082

5(6)-Carboxyfluorescein

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5(6)-Carboxyfluorescein

5(6)-Carboxytetramethylrhodamine Novabiochem®

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5(6)-Carboxytetramethylrhodamine

application(s)

peptide synthesis

application(s)

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

peptide synthesis

storage temp.

2-8°C

storage temp.

-

storage temp.

room temp

storage temp.

2-8°C

Quality Level

200

Quality Level

-

Quality Level

100

Quality Level

200

product line

Novabiochem®

product line

BioReagent

product line

-

product line

Novabiochem®

reaction suitability

reagent type: fluorescent-labelling reagent

reaction suitability

-

reaction suitability

-

reaction suitability

reagent type: fluorescent-labelling reagent

General description

Novabiochem®′s 5(6)-carboxyfluorescein (FAM; λem494 nm; λex518 nm) is a useful tool for preparing fluorescently-labeled peptides. It is supplied as a mixture of 5-and 6-isomers, making it cost effective for those applications which do not require a single isomer dye.

Associated Protocols and Technical Articles
Chromogenic Labeling of Synthetic Peptides

Application

5(6)-Carboxyfluorescein Novabiochem®. CAS 72088-94-9, molar mass 376.32 g/mol.

Linkage

Replaces: 01-63-0149

Analysis Note

Colour (visual): yellow to orange
Appearance of substance (visual): powder
Identity (IR): passes test
Purity (TLC (EtOAc/MeOH/H2O/DIPEA 60 : 30 : 10 : 3)): ≥ 97 %
Assay isomers (HPLC, area%): ≥ 96.0 % (a/a)

To see the solvent systems used for TLC of Novabiochem® products please click here.

Legal Information

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Articles

Chromogenic and fluorogenic derivatives are invaluable tools for biochemistry, having numerous applications in enzymology, protein chemistry, immunology and histochemistry.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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