REST corepressor 1(RCOR1, CoREST) is an essential component of the BHC corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. RCOR1 also promotes the demethylase activity of KDM1A on core histones and nucleosomal substrates and plays a central role in demethylation of Lys-4 of histone H3. Furthermore, RCOR1 is a component of a RCOR/GFI/KDM1A/HDAC complex that suppresses a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. RCOR1 is ubiquitously expressed and is primarily found in the nucleus, but is partially translocated into the cytoplasm upon HSV-1 infection.
This antibody may cross react with human RCOR3 based upon 70% sequence homology.
Recombinant protein corresponding to human Co-Rest/RCOR1.
Anti-Co-Rest/RCOR1 Antibody, clone K72/8 is a highly specific mouse monoclonal antibody, that targets CoRest & has been tested in western blotting, IHC & ICC.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Co-Rest/RCOR1 in human stomach tissue.
Immunocytochemistry Analysis: A representative lot detected Co-Rest/RCOR1 in COS-1 cells expressing Myc-tagged Co-Rest. (Prof. J. Trimmer, University of California, Davis.).
Research Sub Category
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Co-Rest/RCOR1 in 10 µg of Jurkat cell lysate.
~ 65 kDa observed. The calculated molecular weight is 53 kDa, however Co-Rest/RCOR1 has been shown as a ~65-70 kDa band in western blots (You, A., et al. (2001). National Academy of Sciences. 98(4):1454-1458)(Min, G. L., et al. (2005). Nature. 437:432-435).
Protein G Purified
Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Jurkat cell lysate
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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