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SCT064

Sigma-Aldrich

BioTracker NTP-Transporter Molecule

Quick and efficient transport of labeled NTPs into cells for live cell imaging of DNA replication, synthesis, etc. Labeling of DNA with fluorescent NTPs is one of the essential methods in cell cycle analysis.

Synonym(s):
Cell cycle detection, Live cell imaging probe, Live cell imaging probe, Cell cylce probe

assay

≥98% (H-NMR)
≥98% (HPLC)
≥98% (LC/MS)
≥98% (elemental analysis)

technique(s)

cell based assay: suitable

detection method

fluorometric

Related Categories

General description

Nucleoside triphosphates (NTPs) are important metabolites that are involved in numerous cellular processes. However, due to their anionic nature, NTPs do not readily permeate cell membranes. To overcome this barrier, synthetic analogues that are neutral and more readily transportable are commonly used in cell-based assays. However, these synthetic analogues have a very low efficiency of conversion to active metabolites. Hence there is a need to optimize transport of NTPs into the cells.

BioTracker<TMSYMBOL></TMSYMBOL> NTP-Transporter Molecule has a receptor site, for selectively binding to the NTPs, and a cell penetrating agent. The transporter can bind to NTPs and quickly move them into cells without any apparent damage to the cell membrane or decrease in cell viability. The transported NTP is rapidly liberated from the complex and available in the nuclei for incorporation into DNA. Labeled NTPs can be used for live cell imaging, cell proliferation assays, synthetic biology studies, synthesizing artificial DNA, tracking cell division, research on active antiviral and anticancer nucleotides, among other things.

Application

Live cell fluorescent imaging
Quick and efficient transport of labeled NTPs into cells for live cell imaging of DNA replication, synthesis, etc. Labeling of DNA with fluorescent NTPs is one of the essential methods in cell cycle analysis.
Research Category
Cell Imaging
Research Sub Category
Cell Cycle, DNA Replication & Repair

DNA Damage & Repair

Quality

Purity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 3470.14 g/mol

Physical form

Lyophilized

Storage and Stability

Store BioTracker NTP-Transporter Molecule at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Quality

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