MilliporeSigma
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A-005-M

Sigma-Aldrich

Poly-L-Lysine Solution

0.01%

Synonym(s):
Lysine
eCl@ss:
32160801
NACRES:
NA.75

needle size

2.0 mm

Quality Level

sterility

sterile

form

liquid

mol wt

Mw 84000 Da

manufacturer/tradename

NovaSeptum®

concentration

0.01 % (w/v)
0.1 mg/mL

technique(s)

cell attachment: suitable
cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−20°C

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This Item
A-003-MA-004-M457639
form

liquid

form

liquid

form

liquid

form

solid

technique(s)

cell attachment: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell attachment: suitable, cell culture | mammalian: suitable

technique(s)

-

storage temp.

−20°C

storage temp.

-

storage temp.

-

storage temp.

2-8°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

needle size

2.0 mm

needle size

-

needle size

2.0 mm

needle size

-

sterility

sterile

sterility

-

sterility

sterile

sterility

-

General description

Poly-L-Lysine is a synthetic amino acid chain that is positively charged having one hydrobromide per unit of Lysine. Poly-L-Lysine is widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces. Certain cell types secrete proteases, which can digest Poly-L-Lysine.
The molecular weight of Poly-L-Lysine for cell culture can vary significantly, with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (greater than 300,000 Da) having more binding sites per molecule. This product uses a Poly-L-Lysine of 84000 Da, yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment.

Application

Optimal coating concentrations must be determined by end user. Typical coating concentrations range from 10ug/ml to 100ug/ml based on cell type and application.

1. Thaw Poly-L-Lysine solution at room temperature.
2. Dilute Poly-L-Lysine solution to desired concentration in sterile water.
3. Fully coat the cell culture surface with diluted Poly-L-Lysine solution. Use 5 mL volume for 6-cm plates and 10 mL volume for 10-cm plates and T75 flasks.
4. Allow cell culture vessel to sit at room temperature overnight.
5. Aspirate the Poly-L-Lysine solution the following day and rinse vessel with sterile water followed by coating with desired ECM protein.

Quality

Solution is clear with no particulates present. Solution is sterile and suitable for cell culture applications.

Legal Information

NOVASEPTUM is a registered trademark of Millipore AB - Sweden

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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